PDB entry 2d5x

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HEADER    OXYGEN STORAGE/TRANSPORT                08-NOV-05   2D5X              
TITLE     CRYSTAL STRUCTURE OF CARBONMONOXY HORSE HEMOGLOBIN                    
TITLE    2 COMPLEXED WITH L35                                                   
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: HEMOGLOBIN ALPHA SUBUNIT;                                  
COMPND   3 CHAIN: A;                                                            
COMPND   4 SYNONYM: HEMOGLOBIN ALPHA CHAIN, ALPHA-GLOBIN;                       
COMPND   5 MOL_ID: 2;                                                           
COMPND   6 MOLECULE: HEMOGLOBIN BETA SUBUNIT;                                   
COMPND   7 CHAIN: B;                                                            
COMPND   8 SYNONYM: HEMOGLOBIN BETA CHAIN, BETA-GLOBIN                          
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: EQUUS CABALLUS;                                 
SOURCE   3 ORGANISM_COMMON: HORSE;                                              
SOURCE   4 TISSUE: RED CELL;                                                    
SOURCE   5 MOL_ID: 2;                                                           
SOURCE   6 ORGANISM_SCIENTIFIC: EQUUS CABALLUS;                                 
SOURCE   7 ORGANISM_COMMON: HORSE;                                              
SOURCE   8 TISSUE: RED CELL                                                     
KEYWDS    HEMOGLOBIN, L35, ALLOSTERIC EFFECTOR, CRYSTAL STRUCTURE               
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    T.YOKOYAMA,S.NEYA,A.TSUNESHIGE,T.YONETANI,S.Y.PARK,J.R.TAME           
REVDAT   1   07-MAR-06 2D5X    0                                                
JRNL        AUTH   T.YOKOYAMA,S.NEYA,A.TSUNESHIGE,T.YONETANI,S.Y.PARK,          
JRNL        AUTH 2 J.R.TAME                                                     
JRNL        TITL   R-STATE HAEMOGLOBIN WITH LOW OXYGEN AFFINITY:                
JRNL        TITL 2 CRYSTAL STRUCTURES OF DEOXY HUMAN AND CARBONMONOXY           
JRNL        TITL 3 HORSE HAEMOGLOBIN BOUND TO THE EFFECTOR MOLECULE             
JRNL        TITL 4 L35                                                          
JRNL        REF    J.MOL.BIOL.                   V. 356   790 2006              
JRNL        REFN   ASTM JMOBAK  UK ISSN 0022-2836                               
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. 1.45 ANGSTROMS.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : REFMAC 5.2.0003                                      
REMARK   3   AUTHORS     : MURSHUDOV,VAGIN,DODSON                               
REMARK   3                                                                      
REMARK   3    REFINEMENT TARGET : MAXIMUM LIKELIHOOD                            
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.45                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 19.88                          
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 0.000                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 100.0                          
REMARK   3   NUMBER OF REFLECTIONS             : 43215                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT                      
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM                          
REMARK   3   R VALUE     (WORKING + TEST SET) : 0.192                           
REMARK   3   R VALUE            (WORKING SET) : 0.190                           
REMARK   3   FREE R VALUE                     : 0.216                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.000                           
REMARK   3   FREE R VALUE TEST SET COUNT      : 2284                            
REMARK   3                                                                      
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.                                  
REMARK   3   TOTAL NUMBER OF BINS USED           : 20                           
REMARK   3   BIN RESOLUTION RANGE HIGH           : 1.45                         
REMARK   3   BIN RESOLUTION RANGE LOW            : 1.49                         
REMARK   3   REFLECTION IN BIN     (WORKING SET) : 2001                         
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : 100.00                       
REMARK   3   BIN R VALUE           (WORKING SET) : 0.2130                       
REMARK   3   BIN FREE R VALUE SET COUNT          : 80                           
REMARK   3   BIN FREE R VALUE                    : 0.2700                       
REMARK   3                                                                      
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.                    
REMARK   3   ALL ATOMS                : 2567                                    
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 18.45                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : -1.32000                                             
REMARK   3    B22 (A**2) : 0.50000                                              
REMARK   3    B33 (A**2) : 0.82000                                              
REMARK   3    B12 (A**2) : 0.00000                                              
REMARK   3    B13 (A**2) : 0.00000                                              
REMARK   3    B23 (A**2) : 0.00000                                              
REMARK   3                                                                      
REMARK   3  ESTIMATED OVERALL COORDINATE ERROR.                                 
REMARK   3   ESU BASED ON R VALUE                            (A): 0.078         
REMARK   3   ESU BASED ON FREE R VALUE                       (A): 0.078         
REMARK   3   ESU BASED ON MAXIMUM LIKELIHOOD                 (A): 0.050         
REMARK   3   ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): 1.265         
REMARK   3                                                                      
REMARK   3 CORRELATION COEFFICIENTS.                                            
REMARK   3   CORRELATION COEFFICIENT FO-FC      : 0.957                         
REMARK   3   CORRELATION COEFFICIENT FO-FC FREE : 0.942                         
REMARK   3                                                                      
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES        COUNT    RMS    WEIGHT      
REMARK   3   BOND LENGTHS REFINED ATOMS        (A):  2416 ; 0.010 ; 0.021       
REMARK   3   BOND LENGTHS OTHERS               (A):  NULL ;  NULL ;  NULL       
REMARK   3   BOND ANGLES REFINED ATOMS   (DEGREES):  3301 ; 1.250 ; 2.074       
REMARK   3   BOND ANGLES OTHERS          (DEGREES):  NULL ;  NULL ;  NULL       
REMARK   3   TORSION ANGLES, PERIOD 1    (DEGREES):   285 ; 4.506 ; 5.000       
REMARK   3   TORSION ANGLES, PERIOD 2    (DEGREES):    95 ;34.164 ;24.000       
REMARK   3   TORSION ANGLES, PERIOD 3    (DEGREES):   364 ;13.865 ;15.000       
REMARK   3   TORSION ANGLES, PERIOD 4    (DEGREES):     7 ;15.187 ;15.000       
REMARK   3   CHIRAL-CENTER RESTRAINTS       (A**3):   347 ; 0.078 ; 0.200       
REMARK   3   GENERAL PLANES REFINED ATOMS      (A):  1838 ; 0.006 ; 0.020       
REMARK   3   GENERAL PLANES OTHERS             (A):  NULL ;  NULL ;  NULL       
REMARK   3   NON-BONDED CONTACTS REFINED ATOMS (A):  1245 ; 0.228 ; 0.200       
REMARK   3   NON-BONDED CONTACTS OTHERS        (A):  NULL ;  NULL ;  NULL       
REMARK   3   NON-BONDED TORSION REFINED ATOMS  (A):  1671 ; 0.316 ; 0.200       
REMARK   3   NON-BONDED TORSION OTHERS         (A):  NULL ;  NULL ;  NULL       
REMARK   3   H-BOND (X...Y) REFINED ATOMS      (A):   185 ; 0.127 ; 0.200       
REMARK   3   H-BOND (X...Y) OTHERS             (A):  NULL ;  NULL ;  NULL       
REMARK   3   POTENTIAL METAL-ION REFINED ATOMS (A):  NULL ;  NULL ;  NULL       
REMARK   3   POTENTIAL METAL-ION OTHERS        (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY VDW REFINED ATOMS        (A):    53 ; 0.177 ; 0.200       
REMARK   3   SYMMETRY VDW OTHERS               (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY H-BOND REFINED ATOMS     (A):    20 ; 0.153 ; 0.200       
REMARK   3   SYMMETRY H-BOND OTHERS            (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY METAL-ION REFINED ATOMS  (A):  NULL ;  NULL ;  NULL       
REMARK   3   SYMMETRY METAL-ION OTHERS         (A):  NULL ;  NULL ;  NULL       
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.     COUNT   RMS    WEIGHT      
REMARK   3   MAIN-CHAIN BOND REFINED ATOMS  (A**2):  1426 ; 0.655 ; 1.500       
REMARK   3   MAIN-CHAIN BOND OTHER ATOMS    (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   MAIN-CHAIN ANGLE REFINED ATOMS (A**2):  2267 ; 1.238 ; 2.000       
REMARK   3   SIDE-CHAIN BOND REFINED ATOMS  (A**2):  1004 ; 2.005 ; 3.000       
REMARK   3   SIDE-CHAIN ANGLE REFINED ATOMS (A**2):  1034 ; 3.169 ; 4.500       
REMARK   3                                                                      
REMARK   3 ANISOTROPIC THERMAL FACTOR RESTRAINTS.    COUNT   RMS   WEIGHT       
REMARK   3   RIGID-BOND RESTRAINTS          (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SPHERICITY; FREE ATOMS         (A**2):  NULL ;  NULL ;  NULL       
REMARK   3   SPHERICITY; BONDED ATOMS       (A**2):  NULL ;  NULL ;  NULL       
REMARK   3                                                                      
REMARK   3  NCS RESTRAINTS STATISTICS                                           
REMARK   3   NUMBER OF DIFFERENT NCS GROUPS : 0                                 
REMARK   3                                                                      
REMARK   3  TLS DETAILS                                                         
REMARK   3   NUMBER OF TLS GROUPS  : 0                                          
REMARK   3                                                                      
REMARK   3  BULK SOLVENT MODELLING.                                             
REMARK   3   METHOD USED : BABINET MODEL WITH MASK                              
REMARK   3   PARAMETERS FOR MASK CALCULATION                                    
REMARK   3   VDW PROBE RADIUS   : 1.20                                          
REMARK   3   ION PROBE RADIUS   : 0.80                                          
REMARK   3   SHRINKAGE RADIUS   : 0.80                                          
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 2D5X COMPLIES WITH FORMAT V. 3.0, 1-DEC-2006                         
REMARK   4                                                                      
REMARK   4 THIS IS THE REMEDIATED VERSION OF THIS PDB ENTRY.                    
REMARK   4 REMEDIATED DATA FILE REVISION 3.101 (2007-05-01)                     
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ .                              
REMARK 100 THE RCSB ID CODE IS RCSB025026.                                      
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : NULL                               
REMARK 200  TEMPERATURE           (KELVIN) : 100.0                              
REMARK 200  PH                             : 6.00                               
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : PHOTON FACTORY                     
REMARK 200  BEAMLINE                       : AR-NW12A                           
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.000                              
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : CCD                                
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUANTUM 4                     
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : HKL-2000                           
REMARK 200  DATA SCALING SOFTWARE          : SCALEPACK                          
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 45502                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.450                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 20.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 0.000                              
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 87.5                               
REMARK 200  DATA REDUNDANCY                : 3.700                              
REMARK 200  R MERGE                    (I) : 0.05700                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200   FOR THE DATA SET  : 23.3000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.45                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.50                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 56.1                               
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.15800                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200   FOR SHELL         : NULL                               
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT        
REMARK 200 SOFTWARE USED: MOLREP                                                
REMARK 200 STARTING MODEL: NULL                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 47.35                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.34                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 20% PEG1000, 50MM LICL, 50MM MES,        
REMARK 280  0.5MM L35, PH 6.0, BATCH, TEMPERATURE 293K                          
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: C 2 2 21                         
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,-Y,1/2+Z                                             
REMARK 290       3555   -X,Y,1/2-Z                                              
REMARK 290       4555   X,-Y,-Z                                                 
REMARK 290       5555   1/2+X,1/2+Y,Z                                           
REMARK 290       6555   1/2-X,1/2-Y,1/2+Z                                       
REMARK 290       7555   1/2-X,1/2+Y,1/2-Z                                       
REMARK 290       8555   1/2+X,1/2-Y,-Z                                          
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000       43.50800            
REMARK 290   SMTRY1   3 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   3  0.000000  0.000000 -1.000000       43.50800            
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   4  0.000000 -1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   5  1.000000  0.000000  0.000000       31.16850            
REMARK 290   SMTRY2   5  0.000000  1.000000  0.000000       53.72100            
REMARK 290   SMTRY3   5  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   6 -1.000000  0.000000  0.000000       31.16850            
REMARK 290   SMTRY2   6  0.000000 -1.000000  0.000000       53.72100            
REMARK 290   SMTRY3   6  0.000000  0.000000  1.000000       43.50800            
REMARK 290   SMTRY1   7 -1.000000  0.000000  0.000000       31.16850            
REMARK 290   SMTRY2   7  0.000000  1.000000  0.000000       53.72100            
REMARK 290   SMTRY3   7  0.000000  0.000000 -1.000000       43.50800            
REMARK 290   SMTRY1   8  1.000000  0.000000  0.000000       31.16850            
REMARK 290   SMTRY2   8  0.000000 -1.000000  0.000000       53.72100            
REMARK 290   SMTRY3   8  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 THIS ENTRY CONTAINS THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT             
REMARK 300 WHICH CONSISTS OF 2 CHAIN(S). SEE REMARK 350 FOR                     
REMARK 300 INFORMATION ON GENERATING THE BIOLOGICAL MOLECULE(S).                
REMARK 350                                                                      
REMARK 350 GENERATING THE BIOMOLECULE                                           
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B                                  
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 350   BIOMT1   2  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   2  0.000000 -1.000000  0.000000      107.44200            
REMARK 350   BIOMT3   2  0.000000  0.000000 -1.000000       87.01600            
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT                     
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI                             
REMARK 500  FE    HEM B   147     C    CMO    1148              1.68            
REMARK 500  FE    HEM A   142     C    CMO    1143              1.70            
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 2D5Z   RELATED DB: PDB                                   
REMARK 900 CARBONMONOXY HUMAN HEMOGLOBIN COMPLEXED WITH L35                     
REMARK 900 RELATED ID: 2D60   RELATED DB: PDB                                   
REMARK 900 CARBONMONOXY HUMAN HEMOGLOBIN COMPLEXED WITH L35                     
DBREF  2D5X A    1   141  UNP    P01958   HBA_HORSE        1    141             
DBREF  2D5X B    1   146  UNP    P02062   HBB_HORSE        1    146             
SEQRES   1 A  141  VAL LEU SER ALA ALA ASP LYS THR ASN VAL LYS ALA ALA          
SEQRES   2 A  141  TRP SER LYS VAL GLY GLY HIS ALA GLY GLU TYR GLY ALA          
SEQRES   3 A  141  GLU ALA LEU GLU ARG MET PHE LEU GLY PHE PRO THR THR          
SEQRES   4 A  141  LYS THR TYR PHE PRO HIS PHE ASP LEU SER HIS GLY SER          
SEQRES   5 A  141  ALA GLN VAL LYS ALA HIS GLY LYS LYS VAL GLY ASP ALA          
SEQRES   6 A  141  LEU THR LEU ALA VAL GLY HIS LEU ASP ASP LEU PRO GLY          
SEQRES   7 A  141  ALA LEU SER ASN LEU SER ASP LEU HIS ALA HIS LYS LEU          
SEQRES   8 A  141  ARG VAL ASP PRO VAL ASN PHE LYS LEU LEU SER HIS CYS          
SEQRES   9 A  141  LEU LEU SER THR LEU ALA VAL HIS LEU PRO ASN ASP PHE          
SEQRES  10 A  141  THR PRO ALA VAL HIS ALA SER LEU ASP LYS PHE LEU SER          
SEQRES  11 A  141  SER VAL SER THR VAL LEU THR SER LYS TYR ARG                  
SEQRES   1 B  146  VAL GLN LEU SER GLY GLU GLU LYS ALA ALA VAL LEU ALA          
SEQRES   2 B  146  LEU TRP ASP LYS VAL ASN GLU GLU GLU VAL GLY GLY GLU          
SEQRES   3 B  146  ALA LEU GLY ARG LEU LEU VAL VAL TYR PRO TRP THR GLN          
SEQRES   4 B  146  ARG PHE PHE ASP SER PHE GLY ASP LEU SER ASN PRO GLY          
SEQRES   5 B  146  ALA VAL MET GLY ASN PRO LYS VAL LYS ALA HIS GLY LYS          
SEQRES   6 B  146  LYS VAL LEU HIS SER PHE GLY GLU GLY VAL HIS HIS LEU          
SEQRES   7 B  146  ASP ASN LEU LYS GLY THR PHE ALA ALA LEU SER GLU LEU          
SEQRES   8 B  146  HIS CYS ASP LYS LEU HIS VAL ASP PRO GLU ASN PHE ARG          
SEQRES   9 B  146  LEU LEU GLY ASN VAL LEU VAL VAL VAL LEU ALA ARG HIS          
SEQRES  10 B  146  PHE GLY LYS ASP PHE THR PRO GLU LEU GLN ALA SER TYR          
SEQRES  11 B  146  GLN LYS VAL VAL ALA GLY VAL ALA ASN ALA LEU ALA HIS          
SEQRES  12 B  146  LYS TYR HIS                                                  
HET    HEM  A 142      43                                                       
HET    CMO   1143       2                                                       
HET    HEM  B 147      43                                                       
HET    CMO   1148       2                                                       
HET    L35   1200      25                                                       
HET    L35   1201      25                                                       
HETNAM     HEM PROTOPORPHYRIN IX CONTAINING FE                                  
HETNAM     CMO CARBON MONOXIDE                                                  
HETNAM     L35 2-[4-({[(3,5-DICHLOROPHENYL)AMINO]CARBONYL}AMINO)                
HETNAM   2 L35  PHENOXY]-2-METHYLPROPANOIC ACID                                 
HETSYN     HEM HEME                                                             
FORMUL   3  HEM    2(C34 H32 FE N4 O4)                                          
FORMUL   4  CMO    2(C O)                                                       
FORMUL   7  L35    2(C17 H16 CL2 N2 O4)                                         
FORMUL   9  HOH   *224(H2 O)                                                    
HELIX    1   1 SER A    3  VAL A   17  1                                  15    
HELIX    2   2 HIS A   20  PHE A   36  1                                  17    
HELIX    3   3 PRO A   37  PHE A   43  5                                   7    
HELIX    4   4 SER A   52  GLY A   71  1                                  20    
HELIX    5   5 HIS A   72  ASP A   74  5                                   3    
HELIX    6   6 ASP A   75  LEU A   80  1                                   6    
HELIX    7   7 LEU A   80  LYS A   90  1                                  11    
HELIX    8   8 ASP A   94  LEU A  113  1                                  20    
HELIX    9   9 THR A  118  THR A  137  1                                  20    
HELIX   10  10 SER B    4  ASP B   16  1                                  13    
HELIX   11  11 ASN B   19  TYR B   35  1                                  17    
HELIX   12  12 PRO B   36  GLY B   46  5                                  11    
HELIX   13  13 ASN B   50  ASN B   57  1                                   8    
HELIX   14  14 ASN B   57  HIS B   76  1                                  20    
HELIX   15  15 HIS B   77  ASP B   79  5                                   3    
HELIX   16  16 ASN B   80  LYS B   95  1                                  16    
HELIX   17  17 PRO B  100  GLY B  119  1                                  20    
HELIX   18  18 LYS B  120  PHE B  122  5                                   3    
HELIX   19  19 THR B  123  ALA B  142  1                                  20    
HELIX   20  20 HIS B  143  HIS B  146  5                                   4    
LINK         NE2 HIS A  87                FE   HEM A 142                        
LINK         NE2 HIS B  92                FE   HEM B 147                        
CRYST1   62.337  107.442   87.016  90.00  90.00  90.00 C 2 2 21      8          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.016042  0.000000  0.000000        0.00000                         
SCALE2      0.000000  0.009307  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.011492        0.00000