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HEADER OXYGEN STORAGE/TRANSPORT 19-NOV-03 1V4U TITLE CRYSTAL STRUCTURE OF BLUEFIN TUNA CARBONMONOXY-HEMOGLOBIN COMPND MOL_ID: 1; COMPND 2 MOLECULE: HEMOGLOBIN ALPHA CHAIN; COMPND 3 CHAIN: A, C; COMPND 4 MOL_ID: 2; COMPND 5 MOLECULE: HEMOGLOBIN BETA CHAIN; COMPND 6 CHAIN: B, D SOURCE MOL_ID: 1; SOURCE 2 ORGANISM_SCIENTIFIC: THUNNUS THYNNUS; SOURCE 3 ORGANISM_COMMON: BLUEFIN TUNA; SOURCE 4 TISSUE: RED CELL; SOURCE 5 MOL_ID: 2; SOURCE 6 ORGANISM_SCIENTIFIC: THUNNUS THYNNUS; SOURCE 7 ORGANISM_COMMON: BLUEFIN TUNA; SOURCE 8 TISSUE: RED CELL KEYWDS OXYGEN TRANSPORT, HEME, RESPIRATORY PROTEIN, ERYTHROCYTE, KEYWDS 2 ROOT EFFECT, SWIM BLADDER EXPDTA X-RAY DIFFRACTION AUTHOR T.YOKOYAMA,K.T.CHONG,Y.MIYAZAKI,T.NAKATSUKASA,S.UNZAI, AUTHOR 2 G.MIYAZAKI,H.MORIMOTO,R.H.T.JEREMY,S.Y.PARK REVDAT 1 06-JUL-04 1V4U 0 JRNL AUTH T.YOKOYAMA,K.T.CHONG,G.MIYAZAKI,H.MORIMOTO, JRNL AUTH 2 D.T.SHIH,S.UNZAI,J.R.TAME,S.Y.PARK JRNL TITL NOVEL MECHANISMS OF PH SENSITIVITY IN TUNA JRNL TITL 2 HEMOGLOBIN: A STRUCTURAL EXPLANATION OF THE ROOT JRNL TITL 3 EFFECT JRNL REF J.BIOL.CHEM. V. 279 28632 2004 JRNL REFN ASTM JBCHA3 US ISSN 0021-9258 REMARK 1 REMARK 2 REMARK 2 RESOLUTION. 2.00 ANGSTROMS. REMARK 3 REMARK 3 REFINEMENT. REMARK 3 PROGRAM : REFMAC 5.1.22 REMARK 3 AUTHORS : MURSHUDOV,VAGIN,DODSON REMARK 3 REMARK 3 REFINEMENT TARGET : MAXIMUM LIKELIHOOD REMARK 3 REMARK 3 DATA USED IN REFINEMENT. REMARK 3 RESOLUTION RANGE HIGH (ANGSTROMS) : 2.00 REMARK 3 RESOLUTION RANGE LOW (ANGSTROMS) : 20.00 REMARK 3 DATA CUTOFF (SIGMA(F)) : NULL REMARK 3 COMPLETENESS FOR RANGE (%) : 100.0 REMARK 3 NUMBER OF REFLECTIONS : 38274 REMARK 3 REMARK 3 FIT TO DATA USED IN REFINEMENT. REMARK 3 CROSS-VALIDATION METHOD : THROUGHOUT REMARK 3 FREE R VALUE TEST SET SELECTION : RANDOM REMARK 3 R VALUE (WORKING + TEST SET) : 0.207 REMARK 3 R VALUE (WORKING SET) : 0.204 REMARK 3 FREE R VALUE : 0.264 REMARK 3 FREE R VALUE TEST SET SIZE (%) : 5.000 REMARK 3 FREE R VALUE TEST SET COUNT : 2013 REMARK 3 REMARK 3 FIT IN THE HIGHEST RESOLUTION BIN. REMARK 3 TOTAL NUMBER OF BINS USED : 20 REMARK 3 BIN RESOLUTION RANGE HIGH : 2.00 REMARK 3 BIN RESOLUTION RANGE LOW : 2.05 REMARK 3 REFLECTION IN BIN (WORKING SET) : 1941 REMARK 3 BIN COMPLETENESS (WORKING+TEST) (%) : NULL REMARK 3 BIN R VALUE (WORKING SET) : 0.2760 REMARK 3 BIN FREE R VALUE SET COUNT : 113 REMARK 3 BIN FREE R VALUE : 0.3300 REMARK 3 REMARK 3 NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT. REMARK 3 ALL ATOMS : 4694 REMARK 3 REMARK 3 B VALUES. REMARK 3 FROM WILSON PLOT (A**2) : NULL REMARK 3 MEAN B VALUE (OVERALL, A**2) : 30.30 REMARK 3 OVERALL ANISOTROPIC B VALUE. REMARK 3 B11 (A**2) : 1.87000 REMARK 3 B22 (A**2) : -0.51000 REMARK 3 B33 (A**2) : -1.36000 REMARK 3 B12 (A**2) : 0.00000 REMARK 3 B13 (A**2) : 0.00000 REMARK 3 B23 (A**2) : 0.00000 REMARK 3 REMARK 3 ESTIMATED OVERALL COORDINATE ERROR. REMARK 3 ESU BASED ON R VALUE (A): 0.204 REMARK 3 ESU BASED ON FREE R VALUE (A): 0.188 REMARK 3 ESU BASED ON MAXIMUM LIKELIHOOD (A): 0.155 REMARK 3 ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): 5.798 REMARK 3 REMARK 3 CORRELATION COEFFICIENTS. REMARK 3 CORRELATION COEFFICIENT FO-FC : 0.939 REMARK 3 CORRELATION COEFFICIENT FO-FC FREE : 0.908 REMARK 3 REMARK 3 RMS DEVIATIONS FROM IDEAL VALUES COUNT RMS WEIGHT REMARK 3 BOND LENGTHS REFINED ATOMS (A): 4684 ; 0.029 ; 0.022 REMARK 3 BOND LENGTHS OTHERS (A): NULL ; NULL ; NULL REMARK 3 BOND ANGLES REFINED ATOMS (DEGREES): 6396 ; 2.340 ; 2.079 REMARK 3 BOND ANGLES OTHERS (DEGREES): NULL ; NULL ; NULL REMARK 3 TORSION ANGLES, PERIOD 1 (DEGREES): 566 ; 6.928 ; 5.000 REMARK 3 TORSION ANGLES, PERIOD 2 (DEGREES): NULL ; NULL ; NULL REMARK 3 TORSION ANGLES, PERIOD 3 (DEGREES): NULL ; NULL ; NULL REMARK 3 TORSION ANGLES, PERIOD 4 (DEGREES): NULL ; NULL ; NULL REMARK 3 CHIRAL-CENTER RESTRAINTS (A**3): 696 ; 0.193 ; 0.200 REMARK 3 GENERAL PLANES REFINED ATOMS (A): 3492 ; 0.011 ; 0.020 REMARK 3 GENERAL PLANES OTHERS (A): NULL ; NULL ; NULL REMARK 3 NON-BONDED CONTACTS REFINED ATOMS (A): 2441 ; 0.237 ; 0.200 REMARK 3 NON-BONDED CONTACTS OTHERS (A): NULL ; NULL ; NULL REMARK 3 NON-BONDED TORSION REFINED ATOMS (A): NULL ; NULL ; NULL REMARK 3 NON-BONDED TORSION OTHERS (A): NULL ; NULL ; NULL REMARK 3 H-BOND (X...Y) REFINED ATOMS (A): 237 ; 0.167 ; 0.200 REMARK 3 H-BOND (X...Y) OTHERS (A): NULL ; NULL ; NULL REMARK 3 POTENTIAL METAL-ION REFINED ATOMS (A): NULL ; NULL ; NULL REMARK 3 POTENTIAL METAL-ION OTHERS (A): NULL ; NULL ; NULL REMARK 3 SYMMETRY VDW REFINED ATOMS (A): 32 ; 0.269 ; 0.200 REMARK 3 SYMMETRY VDW OTHERS (A): NULL ; NULL ; NULL REMARK 3 SYMMETRY H-BOND REFINED ATOMS (A): 3 ; 0.085 ; 0.200 REMARK 3 SYMMETRY H-BOND OTHERS (A): NULL ; NULL ; NULL REMARK 3 SYMMETRY METAL-ION REFINED ATOMS (A): NULL ; NULL ; NULL REMARK 3 SYMMETRY METAL-ION OTHERS (A): NULL ; NULL ; NULL REMARK 3 REMARK 3 ISOTROPIC THERMAL FACTOR RESTRAINTS. COUNT RMS WEIGHT REMARK 3 MAIN-CHAIN BOND REFINED ATOMS (A**2): 2838 ; 1.270 ; 1.500 REMARK 3 MAIN-CHAIN BOND OTHER ATOMS (A**2): NULL ; NULL ; NULL REMARK 3 MAIN-CHAIN ANGLE REFINED ATOMS (A**2): 4548 ; 2.171 ; 2.000 REMARK 3 SIDE-CHAIN BOND REFINED ATOMS (A**2): 1846 ; 3.597 ; 3.000 REMARK 3 SIDE-CHAIN ANGLE REFINED ATOMS (A**2): 1848 ; 5.250 ; 4.500 REMARK 3 REMARK 3 ANISOTROPIC THERMAL FACTOR RESTRAINTS. COUNT RMS WEIGHT REMARK 3 RIGID-BOND RESTRAINTS (A**2): NULL ; NULL ; NULL REMARK 3 SPHERICITY; FREE ATOMS (A**2): NULL ; NULL ; NULL REMARK 3 SPHERICITY; BONDED ATOMS (A**2): NULL ; NULL ; NULL REMARK 3 REMARK 3 NCS RESTRAINTS STATISTICS REMARK 3 NUMBER OF DIFFERENT NCS GROUPS : 0 REMARK 3 REMARK 3 TLS DETAILS REMARK 3 NUMBER OF TLS GROUPS : 0 REMARK 3 REMARK 3 BULK SOLVENT MODELLING. REMARK 3 METHOD USED : BABINET MODEL WITH MASK REMARK 3 PARAMETERS FOR MASK CALCULATION REMARK 3 VDW PROBE RADIUS : 1.40 REMARK 3 ION PROBE RADIUS : 0.80 REMARK 3 SHRINKAGE RADIUS : 0.80 REMARK 3 REMARK 3 OTHER REFINEMENT REMARKS: NULL REMARK 4 REMARK 4 1V4U COMPLIES WITH FORMAT V. 3.0, 1-DEC-2006 REMARK 4 REMARK 4 THIS IS THE REMEDIATED VERSION OF THIS PDB ENTRY. REMARK 4 REMEDIATED DATA FILE REVISION 3.100 (2007-03-17) REMARK 100 REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ . REMARK 100 THE RCSB ID CODE IS RCSB006207. REMARK 200 REMARK 200 EXPERIMENTAL DETAILS REMARK 200 EXPERIMENT TYPE : X-RAY DIFFRACTION REMARK 200 DATE OF DATA COLLECTION : NULL REMARK 200 TEMPERATURE (KELVIN) : 100.0 REMARK 200 PH : 7.50 REMARK 200 NUMBER OF CRYSTALS USED : 1 REMARK 200 REMARK 200 SYNCHROTRON (Y/N) : Y REMARK 200 RADIATION SOURCE : SPRING-8 REMARK 200 BEAMLINE : BL44B2 REMARK 200 X-RAY GENERATOR MODEL : NULL REMARK 200 MONOCHROMATIC OR LAUE (M/L) : M REMARK 200 WAVELENGTH OR RANGE (A) : 1.0000 REMARK 200 MONOCHROMATOR : NULL REMARK 200 OPTICS : NULL REMARK 200 REMARK 200 DETECTOR TYPE : CCD REMARK 200 DETECTOR MANUFACTURER : MARRESEARCH REMARK 200 INTENSITY-INTEGRATION SOFTWARE : HKL-2000 REMARK 200 DATA SCALING SOFTWARE : SCALEPACK REMARK 200 REMARK 200 NUMBER OF UNIQUE REFLECTIONS : 40369 REMARK 200 RESOLUTION RANGE HIGH (A) : 2.000 REMARK 200 RESOLUTION RANGE LOW (A) : 20.000 REMARK 200 REJECTION CRITERIA (SIGMA(I)) : 0.000 REMARK 200 REMARK 200 OVERALL. REMARK 200 COMPLETENESS FOR RANGE (%) : 89.1 REMARK 200 DATA REDUNDANCY : 2.900 REMARK 200 R MERGE (I) : 0.03600 REMARK 200 R SYM (I) : NULL REMARK 200 FOR THE DATA SET : NULL REMARK 200 REMARK 200 IN THE HIGHEST RESOLUTION SHELL. REMARK 200 HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 2.00 REMARK 200 HIGHEST RESOLUTION SHELL, RANGE LOW (A) : 2.07 REMARK 200 COMPLETENESS FOR SHELL (%) : 64.7 REMARK 200 DATA REDUNDANCY IN SHELL : NULL REMARK 200 R MERGE FOR SHELL (I) : 0.14100 REMARK 200 R SYM FOR SHELL (I) : NULL REMARK 200 FOR SHELL : NULL REMARK 200 REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT REMARK 200 SOFTWARE USED: MOLREP REMARK 200 STARTING MODEL: NULL REMARK 200 REMARK 200 REMARK: NULL REMARK 280 REMARK 280 CRYSTAL REMARK 280 SOLVENT CONTENT, VS (%): 52.48 REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.59 REMARK 280 REMARK 280 CRYSTALLIZATION CONDITIONS: PEG 2000, SODIUM PHOSPHATE, PH 7.5, REMARK 280 BATCH METHOD, TEMPERATURE 293K REMARK 290 REMARK 290 CRYSTALLOGRAPHIC SYMMETRY REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 21 REMARK 290 REMARK 290 SYMOP SYMMETRY REMARK 290 NNNMMM OPERATOR REMARK 290 1555 X,Y,Z REMARK 290 2555 1/2-X,-Y,1/2+Z REMARK 290 3555 -X,1/2+Y,1/2-Z REMARK 290 4555 1/2+X,1/2-Y,-Z REMARK 290 REMARK 290 WHERE NNN -> OPERATOR NUMBER REMARK 290 MMM -> TRANSLATION VECTOR REMARK 290 REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY REMARK 290 RELATED MOLECULES. REMARK 290 SMTRY1 1 1.000000 0.000000 0.000000 0.00000 REMARK 290 SMTRY2 1 0.000000 1.000000 0.000000 0.00000 REMARK 290 SMTRY3 1 0.000000 0.000000 1.000000 0.00000 REMARK 290 SMTRY1 2 -1.000000 0.000000 0.000000 29.50800 REMARK 290 SMTRY2 2 0.000000 -1.000000 0.000000 0.00000 REMARK 290 SMTRY3 2 0.000000 0.000000 1.000000 54.22550 REMARK 290 SMTRY1 3 -1.000000 0.000000 0.000000 0.00000 REMARK 290 SMTRY2 3 0.000000 1.000000 0.000000 51.27050 REMARK 290 SMTRY3 3 0.000000 0.000000 -1.000000 54.22550 REMARK 290 SMTRY1 4 1.000000 0.000000 0.000000 29.50800 REMARK 290 SMTRY2 4 0.000000 -1.000000 0.000000 51.27050 REMARK 290 SMTRY3 4 0.000000 0.000000 -1.000000 0.00000 REMARK 290 REMARK 290 REMARK: NULL REMARK 300 REMARK 300 BIOMOLECULE: 1 REMARK 300 THIS ENTRY CONTAINS THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT REMARK 300 WHICH CONSISTS OF 4 CHAIN(S). SEE REMARK 350 FOR REMARK 300 INFORMATION ON GENERATING THE BIOLOGICAL MOLECULE(S). REMARK 350 REMARK 350 GENERATING THE BIOMOLECULE REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS REMARK 350 GIVEN BELOW. BOTH NON-CRYSTALLOGRAPHIC AND REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN. REMARK 350 REMARK 350 BIOMOLECULE: 1 REMARK 350 APPLY THE FOLLOWING TO CHAINS: A, B, C, D REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000 REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000 REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000 REMARK 465 REMARK 465 MISSING RESIDUES REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.) REMARK 465 REMARK 465 M RES C SSSEQI REMARK 465 ARG B 143 REMARK 465 LYS B 144 REMARK 465 TYR B 145 REMARK 465 HIS B 146 REMARK 465 ARG D 143 REMARK 465 LYS D 144 REMARK 465 TYR D 145 REMARK 465 HIS D 146 REMARK 500 REMARK 500 GEOMETRY AND STEREOCHEMISTRY REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT REMARK 500 REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT. REMARK 500 REMARK 500 ATM1 RES C SSEQI ATM2 RES C SSEQI REMARK 500 ND HEM C 144 C CMO C 145 2.19 REMARK 500 REMARK 500 GEOMETRY AND STEREOCHEMISTRY REMARK 500 SUBTOPIC: COVALENT BOND LENGTHS REMARK 500 REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE). REMARK 500 REMARK 500 STANDARD TABLE: REMARK 500 FORMAT: (10X,I3,1X,2(A3,1X,A1,I4,A1,1X,A4,3X),F6.3) REMARK 500 REMARK 500 EXPECTED VALUES: ENGH AND HUBER, 1991 REMARK 500 REMARK 500 M RES CSSEQI ATM1 RES CSSEQI ATM2 DEVIATION REMARK 500 MET A 50 SD MET A 50 CE -0.205 REMARK 500 MET C 50 SD MET C 50 CE -0.177 REMARK 900 REMARK 900 RELATED ENTRIES REMARK 900 RELATED ID: 1V4W RELATED DB: PDB REMARK 900 BLUEFIN TUNA HEMOGLOBIN DEOXY FORM AT PH7.5, AT 1.70 A REMARK 900 RELATED ID: 1V4X RELATED DB: PDB REMARK 900 BLUEFIN TUNA HEMOGLOBIN DEOXY FORM AT PH5.0, AT 1.60 A DBREF 1V4U A 1 143 UNP Q8AYM0 Q8AYM0_THUTH 2 144 DBREF 1V4U B 1 146 UNP Q8AYM1 Q8AYM1_THUTH 2 147 DBREF 1V4U C 1 143 UNP Q8AYM0 Q8AYM0_THUTH 2 144 DBREF 1V4U D 1 146 UNP Q8AYM1 Q8AYM1_THUTH 2 147 SEQRES 1 A 143 THR THR LEU SER ASP LYS ASP LYS SER THR VAL LYS ALA SEQRES 2 A 143 LEU TRP GLY LYS ILE SER LYS SER ALA ASP ALA ILE GLY SEQRES 3 A 143 ALA ASP ALA LEU GLY ARG MET LEU ALA VAL TYR PRO GLN SEQRES 4 A 143 THR LYS THR TYR PHE SER HIS TRP PRO ASP MET SER PRO SEQRES 5 A 143 GLY SER GLY PRO VAL LYS ALA HIS GLY LYS LYS VAL MET SEQRES 6 A 143 GLY GLY VAL ALA LEU ALA VAL SER LYS ILE ASP ASP LEU SEQRES 7 A 143 THR THR GLY LEU GLY ASP LEU SER GLU LEU HIS ALA PHE SEQRES 8 A 143 LYS MET ARG VAL ASP PRO SER ASN PHE LYS ILE LEU SER SEQRES 9 A 143 HIS CYS ILE LEU VAL VAL VAL ALA LYS MET PHE PRO LYS SEQRES 10 A 143 GLU PHE THR PRO ASP ALA HIS VAL SER LEU ASP LYS PHE SEQRES 11 A 143 LEU ALA SER VAL ALA LEU ALA LEU ALA GLU ARG TYR ARG SEQRES 1 B 146 VAL GLU TRP THR GLN GLN GLU ARG SER ILE ILE ALA GLY SEQRES 2 B 146 ILE PHE ALA ASN LEU ASN TYR GLU ASP ILE GLY PRO LYS SEQRES 3 B 146 ALA LEU ALA ARG CYS LEU ILE VAL TYR PRO TRP THR GLN SEQRES 4 B 146 ARG TYR PHE GLY ALA TYR GLY ASP LEU SER THR PRO ASP SEQRES 5 B 146 ALA ILE LYS GLY ASN ALA LYS ILE ALA ALA HIS GLY VAL SEQRES 6 B 146 LYS VAL LEU HIS GLY LEU ASP ARG ALA VAL LYS ASN MET SEQRES 7 B 146 ASP ASN ILE ASN GLU ALA TYR SER GLU LEU SER VAL LEU SEQRES 8 B 146 HIS SER ASP LYS LEU HIS VAL ASP PRO ASP ASN PHE ARG SEQRES 9 B 146 ILE LEU GLY ASP CYS LEU THR VAL VAL ILE ALA ALA ASN SEQRES 10 B 146 LEU GLY ASP ALA PHE THR VAL GLU THR GLN CYS ALA PHE SEQRES 11 B 146 GLN LYS PHE LEU ALA VAL VAL VAL PHE ALA LEU GLY ARG SEQRES 12 B 146 LYS TYR HIS SEQRES 1 C 143 THR THR LEU SER ASP LYS ASP LYS SER THR VAL LYS ALA SEQRES 2 C 143 LEU TRP GLY LYS ILE SER LYS SER ALA ASP ALA ILE GLY SEQRES 3 C 143 ALA ASP ALA LEU GLY ARG MET LEU ALA VAL TYR PRO GLN SEQRES 4 C 143 THR LYS THR TYR PHE SER HIS TRP PRO ASP MET SER PRO SEQRES 5 C 143 GLY SER GLY PRO VAL LYS ALA HIS GLY LYS LYS VAL MET SEQRES 6 C 143 GLY GLY VAL ALA LEU ALA VAL SER LYS ILE ASP ASP LEU SEQRES 7 C 143 THR THR GLY LEU GLY ASP LEU SER GLU LEU HIS ALA PHE SEQRES 8 C 143 LYS MET ARG VAL ASP PRO SER ASN PHE LYS ILE LEU SER SEQRES 9 C 143 HIS CYS ILE LEU VAL VAL VAL ALA LYS MET PHE PRO LYS SEQRES 10 C 143 GLU PHE THR PRO ASP ALA HIS VAL SER LEU ASP LYS PHE SEQRES 11 C 143 LEU ALA SER VAL ALA LEU ALA LEU ALA GLU ARG TYR ARG SEQRES 1 D 146 VAL GLU TRP THR GLN GLN GLU ARG SER ILE ILE ALA GLY SEQRES 2 D 146 ILE PHE ALA ASN LEU ASN TYR GLU ASP ILE GLY PRO LYS SEQRES 3 D 146 ALA LEU ALA ARG CYS LEU ILE VAL TYR PRO TRP THR GLN SEQRES 4 D 146 ARG TYR PHE GLY ALA TYR GLY ASP LEU SER THR PRO ASP SEQRES 5 D 146 ALA ILE LYS GLY ASN ALA LYS ILE ALA ALA HIS GLY VAL SEQRES 6 D 146 LYS VAL LEU HIS GLY LEU ASP ARG ALA VAL LYS ASN MET SEQRES 7 D 146 ASP ASN ILE ASN GLU ALA TYR SER GLU LEU SER VAL LEU SEQRES 8 D 146 HIS SER ASP LYS LEU HIS VAL ASP PRO ASP ASN PHE ARG SEQRES 9 D 146 ILE LEU GLY ASP CYS LEU THR VAL VAL ILE ALA ALA ASN SEQRES 10 D 146 LEU GLY ASP ALA PHE THR VAL GLU THR GLN CYS ALA PHE SEQRES 11 D 146 GLN LYS PHE LEU ALA VAL VAL VAL PHE ALA LEU GLY ARG SEQRES 12 D 146 LYS TYR HIS HET ACE A 0 3 HET ACE C 0 3 HET HEM A 144 43 HET CMO A 145 2 HET HEM B 147 43 HET CMO B 148 2 HET HEM C 144 43 HET CMO C 145 2 HET HEM D 147 43 HET CMO D 148 2 HETNAM ACE ACETYL GROUP HETNAM HEM PROTOPORPHYRIN IX CONTAINING FE HETNAM CMO CARBON MONOXIDE HETSYN HEM HEME FORMUL 5 ACE 2(C2 H4 O) FORMUL 7 HEM 4(C34 H32 FE N4 O4) FORMUL 8 CMO 4(C O) FORMUL 15 HOH *138(H2 O) HELIX 1 1 SER A 4 SER A 19 1 16 HELIX 2 2 SER A 21 TYR A 37 1 17 HELIX 3 3 PRO A 38 SER A 45 5 8 HELIX 4 4 SER A 54 LYS A 74 1 21 HELIX 5 5 ASP A 77 LEU A 82 1 6 HELIX 6 6 LEU A 82 PHE A 91 1 10 HELIX 7 7 PRO A 97 PHE A 115 1 19 HELIX 8 8 THR A 120 ALA A 139 1 20 HELIX 9 9 THR B 4 LEU B 18 1 15 HELIX 10 10 ASN B 19 TYR B 35 1 17 HELIX 11 11 PRO B 36 GLY B 46 5 11 HELIX 12 12 THR B 50 LYS B 55 1 6 HELIX 13 13 ASN B 57 ASN B 77 1 21 HELIX 14 14 ASN B 80 ASP B 94 1 15 HELIX 15 15 PRO B 100 GLY B 119 1 20 HELIX 16 16 ASP B 120 PHE B 122 5 3 HELIX 17 17 THR B 123 GLY B 142 1 20 HELIX 18 18 SER C 4 SER C 19 1 16 HELIX 19 19 SER C 21 TYR C 37 1 17 HELIX 20 20 PRO C 38 PHE C 44 5 7 HELIX 21 21 SER C 54 LYS C 74 1 21 HELIX 22 22 ASP C 77 LEU C 82 1 6 HELIX 23 23 LEU C 82 PHE C 91 1 10 HELIX 24 24 PRO C 97 PHE C 115 1 19 HELIX 25 25 THR C 120 ALA C 139 1 20 HELIX 26 26 GLU C 140 ARG C 143 5 4 HELIX 27 27 THR D 4 LEU D 18 1 15 HELIX 28 28 ASN D 19 TYR D 35 1 17 HELIX 29 29 PRO D 36 GLY D 46 5 11 HELIX 30 30 THR D 50 ASN D 57 1 8 HELIX 31 31 ASN D 57 LYS D 76 1 20 HELIX 32 32 ASN D 80 TYR D 85 1 6 HELIX 33 33 TYR D 85 SER D 93 1 9 HELIX 34 34 ASP D 99 GLY D 119 1 21 HELIX 35 35 ASP D 120 PHE D 122 5 3 HELIX 36 36 THR D 123 GLY D 142 1 20 LINK FE HEM A 144 NE2 HIS A 89 LINK FE HEM B 147 NE2 HIS B 92 LINK FE HEM C 144 NE2 HIS C 89 LINK FE HEM D 147 NE2 HIS D 92 LINK C CMO A 145 FE HEM A 144 LINK C CMO B 148 FE HEM B 147 LINK C CMO C 145 FE HEM C 144 LINK C CMO D 148 FE HEM D 147 LINK C ACE A 0 N THR A 1 LINK C ACE C 0 N THR C 1 CRYST1 59.016 102.541 108.451 90.00 90.00 90.00 P 21 21 21 8 ORIGX1 1.000000 0.000000 0.000000 0.00000 ORIGX2 0.000000 1.000000 0.000000 0.00000 ORIGX3 0.000000 0.000000 1.000000 0.00000 SCALE1 0.016945 0.000000 0.000000 0.00000 SCALE2 0.000000 0.009752 0.000000 0.00000 SCALE3 0.000000 0.000000 0.009221 0.00000