PDB entry 1o2g

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HEADER    BLOOD CLOTTING, HYDROLASE               06-MAR-03   1O2G              
TITLE     ELABORATE MANIFOLD OF SHORT HYDROGEN BOND ARRAYS MEDIATING            
TITLE    2 BINDING OF ACTIVE SITE-DIRECTED SERINE PROTEASE INHIBITORS           
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: THROMBIN;                                                  
COMPND   3 CHAIN: L;                                                            
COMPND   4 FRAGMENT: LIGHT CHAIN, RESIDUES 328-363;                             
COMPND   5 SYNONYM: COAGULATION FACTOR II;                                      
COMPND   6 EC: 3.4.21.5;                                                        
COMPND   7 MOL_ID: 2;                                                           
COMPND   8 MOLECULE: THROMBIN;                                                  
COMPND   9 CHAIN: H;                                                            
COMPND  10 FRAGMENT: HEAVY CHAIN, RESIDUES 364-620;                             
COMPND  11 SYNONYM: COAGULATION FACTOR II;                                      
COMPND  12 EC: 3.4.21.5;                                                        
COMPND  13 MOL_ID: 3;                                                           
COMPND  14 MOLECULE: ACETYL HIRUDIN;                                            
COMPND  15 CHAIN: I;                                                            
COMPND  16 ENGINEERED: YES                                                      
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 MOL_ID: 2;                                                           
SOURCE   5 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   6 ORGANISM_COMMON: HUMAN;                                              
SOURCE   7 MOL_ID: 3;                                                           
SOURCE   8 SYNTHETIC: YES                                                       
KEYWDS    SERINE PROTEASE, SHORT HYDROGEN BOND, INHIBITION MECHANISM,           
KEYWDS   2 SHIFT OF PKA, TRYPSIN, THROMBIN, UROKINASE, FACTOR XA                
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    B.A.KATZ,K.ELROD,E.VERNER,R.L.MACKMAN,C.LUONG,W.SHRADER,              
AUTHOR   2 M.SENDZIK,J.R.SPENCER,P.A.SPRENGELER,A.KOLESNIKOV,W.F.TAI,           
AUTHOR   3 H.HUI,G.BREITENBUCHER,D.ALLEN,J.JANC                                 
REVDAT   1   13-MAY-03 1O2G    0                                                
JRNL        AUTH   B.A.KATZ,K.ELROD,E.VERNER,R.L.MACKMAN,C.LUONG,               
JRNL        AUTH 2 W.D.SHRADER,M.SENDZIK,J.R.SPENCER,P.A.SPRENGELER,            
JRNL        AUTH 3 A.KOLESNIKOV,V.W.-F.TAI,H.C.HUI,J.G.BREITENBUCHER,           
JRNL        AUTH 4 D.ALLEN,J.W.JANC                                             
JRNL        TITL   ELABORATE MANIFOLD OF SHORT HYDROGEN BOND ARRAYS             
JRNL        TITL 2 MEDIATING BINDING OF ACTIVE SITE-DIRECTED SERINE             
JRNL        TITL 3 PROTEASE INHIBITORS                                          
JRNL        REF    J.MOL.BIOL.                   V. 329    93 2003              
JRNL        REFN   ASTM JMOBAK  UK ISSN 0022-2836                               
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. 1.58 ANGSTROMS.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : X-PLOR 3.851                                         
REMARK   3   AUTHORS     : BRUNGER                                              
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.58                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 7.00                           
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 0.700                          
REMARK   3   DATA CUTOFF HIGH         (ABS(F)) : NULL                           
REMARK   3   DATA CUTOFF LOW          (ABS(F)) : NULL                           
REMARK   3   COMPLETENESS (WORKING+TEST)   (%) : 87.3                           
REMARK   3   NUMBER OF REFLECTIONS             : 42749                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT                      
REMARK   3   FREE R VALUE TEST SET SELECTION  : NULL                            
REMARK   3   R VALUE            (WORKING SET) : 0.219                           
REMARK   3   FREE R VALUE                     : 0.250                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 10.000                          
REMARK   3   FREE R VALUE TEST SET COUNT      : 4349                            
REMARK   3   ESTIMATED ERROR OF FREE R VALUE  : NULL                            
REMARK   3                                                                      
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.                                  
REMARK   3   TOTAL NUMBER OF BINS USED           : NULL                         
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : NULL                         
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : NULL                         
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : NULL                         
REMARK   3   REFLECTIONS IN BIN    (WORKING SET) : NULL                         
REMARK   3   BIN R VALUE           (WORKING SET) : NULL                         
REMARK   3   BIN FREE R VALUE                    : NULL                         
REMARK   3   BIN FREE R VALUE TEST SET SIZE  (%) : NULL                         
REMARK   3   BIN FREE R VALUE TEST SET COUNT     : NULL                         
REMARK   3   ESTIMATED ERROR OF BIN FREE R VALUE : NULL                         
REMARK   3                                                                      
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.                    
REMARK   3   PROTEIN ATOMS            : 4842                                    
REMARK   3   NUCLEIC ACID ATOMS       : 0                                       
REMARK   3   HETEROGEN ATOMS          : 43                                      
REMARK   3   SOLVENT ATOMS            : 930                                     
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : NULL                           
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : NULL                           
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  ESTIMATED COORDINATE ERROR.                                         
REMARK   3   ESD FROM LUZZATI PLOT        (A) : NULL                            
REMARK   3   ESD FROM SIGMAA              (A) : NULL                            
REMARK   3   LOW RESOLUTION CUTOFF        (A) : NULL                            
REMARK   3                                                                      
REMARK   3  CROSS-VALIDATED ESTIMATED COORDINATE ERROR.                         
REMARK   3   ESD FROM C-V LUZZATI PLOT    (A) : NULL                            
REMARK   3   ESD FROM C-V SIGMAA          (A) : NULL                            
REMARK   3                                                                      
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES.                                   
REMARK   3   BOND LENGTHS                 (A) : 0.018                           
REMARK   3   BOND ANGLES            (DEGREES) : 3.70                            
REMARK   3   DIHEDRAL ANGLES        (DEGREES) : NULL                            
REMARK   3   IMPROPER ANGLES        (DEGREES) : NULL                            
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL MODEL : NULL                                      
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.    RMS    SIGMA                
REMARK   3   MAIN-CHAIN BOND              (A**2) : NULL  ; NULL                 
REMARK   3   MAIN-CHAIN ANGLE             (A**2) : NULL  ; NULL                 
REMARK   3   SIDE-CHAIN BOND              (A**2) : NULL  ; NULL                 
REMARK   3   SIDE-CHAIN ANGLE             (A**2) : NULL  ; NULL                 
REMARK   3                                                                      
REMARK   3  NCS MODEL : NULL                                                    
REMARK   3                                                                      
REMARK   3  NCS RESTRAINTS.                         RMS   SIGMA/WEIGHT          
REMARK   3   GROUP  1  POSITIONAL            (A) : NULL  ; NULL                 
REMARK   3   GROUP  1  B-FACTOR           (A**2) : NULL  ; NULL                 
REMARK   3                                                                      
REMARK   3  PARAMETER FILE  1  : NULL                                           
REMARK   3  TOPOLOGY FILE  1   : NULL                                           
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: MET_H84, LEU_H130, VAL_H157, PRO_         
REMARK   3  H166, AND ILE_H174 WERE SIMULTANEOUSLY REFINED IN TWO               
REMARK   3  CONFORMATIONS. NO DENSITY WAS OBSERVED FOR THR147, TRP148,          
REMARK   3  THR149, ALA149A, ASN149B, VAL149C, GLY149D, AND LYS149E IN THE      
REMARK   3  AUTOLYSIS LOOP, AND THESE RESIDUES ARE NOT INCLUDED IN THE          
REMARK   3  MODEL. IN THE NON-ACTIVE SITE PEPTIDE INHIBITOR                     
REMARK   3  (ACETYLHIRUDIN) THE TYROSINE HYDROXYL IS SULFONATED. HOH477,        
REMARK   3  OGSER195 AND O6' OF THE INHIBITOR MAKE A 3-CENTERED SHORT           
REMARK   3  HYDROGEN BOND ARRAY. DISORDERED WATERS INCLUDE: HOH395 IS           
REMARK   3  CLOSE TO A SYMMETRY RELATED EQUIVALENT OF ITSELF. HOH396 IS         
REMARK   3  CLOSE TO A SYMMETRY RELATED EQUIVALENT OF ITSELF. HOH397 IS         
REMARK   3  CLOSE TO A SYMMETRY RELATED EQUIVALENT OF ITSELF. HIS_H57 IS        
REMARK   3  DOUBLY PROTONATED. HIS_H91 AND HIS_H119 ARE MONOPROTONATED ON       
REMARK   3  THE EPSILON NITROGEN                                                
REMARK   4                                                                      
REMARK   4 1O2G COMPLIES WITH FORMAT V. 3.0, 1-DEC-2006                         
REMARK   4                                                                      
REMARK   4 THIS IS THE REMEDIATED VERSION OF THIS PDB ENTRY.                    
REMARK   4 REMEDIATED DATA FILE REVISION 3.101 (2007-05-29)                     
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY RCSB.                               
REMARK 100 THE RCSB ID CODE IS RCSB001720.                                      
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 04-JUN-2002                        
REMARK 200  TEMPERATURE           (KELVIN) : 285.0                              
REMARK 200  PH                             : 6.50                               
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : N                                  
REMARK 200  RADIATION SOURCE               : ROTATING ANODE                     
REMARK 200  BEAMLINE                       : NULL                               
REMARK 200  X-RAY GENERATOR MODEL          : RIGAKU RUH3R                       
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.5418                             
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : IMAGE PLATE                        
REMARK 200  DETECTOR MANUFACTURER          : RIGAKU RAXIS IV++                  
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : CRYSTALCLEAR (MSC/RIGAKU)          
REMARK 200  DATA SCALING SOFTWARE          : CRYSTALCLEAR (MSC/RIGAKU)          
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 45048                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.580                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 32.190                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 0.000                              
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 90.9                               
REMARK 200  DATA REDUNDANCY                : 2.000                              
REMARK 200  R MERGE                    (I) : 0.04600                            
REMARK 200  R SYM                      (I) : NULL                               
REMARK 200   FOR THE DATA SET  : 9.6000                             
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.58                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.65                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : NULL                               
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.36000                            
REMARK 200  R SYM FOR SHELL            (I) : NULL                               
REMARK 200   FOR SHELL         : 1.200                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: FOURIER SYNTHESIS            
REMARK 200 SOFTWARE USED: QUANTA                                                
REMARK 200 STARTING MODEL: NULL                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 52.60                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.59                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: 2-PROPANOL, PEG 4000, PH 6.5, VAPOR      
REMARK 280  DIFFUSION AT 298 K, PH 6.50                                         
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: C 1 2 1                          
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,Y,-Z                                                 
REMARK 290       3555   1/2+X,1/2+Y,Z                                           
REMARK 290       4555   1/2-X,1/2+Y,-Z                                          
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   3  1.000000  0.000000  0.000000       35.68000            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       36.03000            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000       35.68000            
REMARK 290   SMTRY2   4  0.000000  1.000000  0.000000       36.03000            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 THIS ENTRY CONTAINS THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT             
REMARK 300 WHICH CONSISTS OF 3 CHAIN(S). SEE REMARK 350 FOR                     
REMARK 300 INFORMATION ON GENERATING THE BIOLOGICAL MOLECULE(S).                
REMARK 350                                                                      
REMARK 350 GENERATING THE BIOMOLECULE                                           
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 APPLY THE FOLLOWING TO CHAINS: L, H, I                               
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 465                                                                      
REMARK 465 MISSING RESIDUES                                                     
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE                       
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)                
REMARK 465                                                                      
REMARK 465   M RES C SSSEQI                                                     
REMARK 465     THR H   146A                                                     
REMARK 465     TRP H   146B                                                     
REMARK 465     THR H   146C                                                     
REMARK 465     ALA H   146D                                                     
REMARK 465     ASN H   146E                                                     
REMARK 465     VAL H   146F                                                     
REMARK 465     GLY H   146G                                                     
REMARK 465     LYS H   146H                                                     
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT                     
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI                             
REMARK 500   OG   SER H   195     O6'  696     246              2.14            
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS                                             
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS THAT ARE RELATED BY CRYSTALLOGRAPHIC             
REMARK 500 SYMMETRY ARE IN CLOSE CONTACT.  AN ATOM LOCATED WITHIN 0.15          
REMARK 500 ANGSTROMS OF A SYMMETRY RELATED ATOM IS ASSUMED TO BE ON A           
REMARK 500 SPECIAL POSITION AND IS, THEREFORE, LISTED IN REMARK 375             
REMARK 500 INSTEAD OF REMARK 500.  ATOMS WITH NON-BLANK ALTERNATE               
REMARK 500 LOCATION INDICATORS ARE NOT INCLUDED IN THE CALCULATIONS.            
REMARK 500                                                                      
REMARK 500 DISTANCE CUTOFF:                                                     
REMARK 500 2.2 ANGSTROMS FOR CONTACTS NOT INVOLVING HYDROGEN ATOMS              
REMARK 500 1.6 ANGSTROMS FOR CONTACTS INVOLVING HYDROGEN ATOMS                  
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI  SSYMOP   DISTANCE          
REMARK 500   O    ARG H    77A    O    HOH     395     2555     1.80            
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: ENGH AND HUBER, 1991                                
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500    SER L   1E  N   -  CA  -  C   ANGL. DEV. =-35.2 DEGREES           
REMARK 500    GLY L   1D  N   -  CA  -  C   ANGL. DEV. = 18.3 DEGREES           
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: TORSION ANGLES                                             
REMARK 500                                                                      
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:            
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;               
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                             
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)                    
REMARK 500                                                                      
REMARK 500  M RES CSSEQI        PSI       PHI                                   
REMARK 500    SER L   1E     -80.38     88.69                                   
REMARK 500    ALA L   1B     -59.50    127.40                                   
REMARK 500    ASP L  14L     160.27     81.48                                   
REMARK 525                                                                      
REMARK 525 SOLVENT                                                              
REMARK 525 THE FOLLOWING SOLVENT MOLECULES LIE FARTHER THAN EXPECTED            
REMARK 525 FROM THE PROTEIN OR NUCLEIC ACID MOLECULE AND MAY BE                 
REMARK 525 ASSOCIATED WITH A SYMMETRY RELATED MOLECULE (M=MODEL                 
REMARK 525 NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE          
REMARK 525 NUMBER; I=INSERTION CODE):                                           
REMARK 525                                                                      
REMARK 525  M RES CSSEQI                                                        
REMARK 525    HOH   467        DISTANCE =  5.01 ANGSTROMS                       
REMARK 525    HOH   479        DISTANCE =  5.63 ANGSTROMS                       
REMARK 525    HOH   495        DISTANCE =  5.63 ANGSTROMS                       
REMARK 525    HOH   529        DISTANCE =  8.02 ANGSTROMS                       
REMARK 525    HOH   697        DISTANCE =  6.29 ANGSTROMS                       
REMARK 525    HOH   727        DISTANCE =  5.05 ANGSTROMS                       
REMARK 525    HOH   785        DISTANCE =  6.95 ANGSTROMS                       
REMARK 525    HOH   793        DISTANCE =  6.44 ANGSTROMS                       
REMARK 525    HOH   800        DISTANCE =  6.80 ANGSTROMS                       
REMARK 525    HOH   802        DISTANCE =  5.97 ANGSTROMS                       
REMARK 525    HOH   834        DISTANCE =  5.23 ANGSTROMS                       
REMARK 525    HOH   858        DISTANCE =  5.77 ANGSTROMS                       
REMARK 525    HOH   865        DISTANCE =  6.26 ANGSTROMS                       
REMARK 525    HOH   873        DISTANCE =  9.95 ANGSTROMS                       
REMARK 525    HOH   879        DISTANCE =  5.51 ANGSTROMS                       
REMARK 525    HOH   888        DISTANCE =  6.01 ANGSTROMS                       
REMARK 525    HOH   910        DISTANCE = 11.76 ANGSTROMS                       
REMARK 525    HOH  1034        DISTANCE =  5.78 ANGSTROMS                       
REMARK 525    HOH  1137        DISTANCE =  7.39 ANGSTROMS                       
REMARK 525    HOH  1147        DISTANCE =  5.47 ANGSTROMS                       
REMARK 525    HOH  1166        DISTANCE =  5.90 ANGSTROMS                       
REMARK 525    HOH  1200        DISTANCE =  6.68 ANGSTROMS                       
REMARK 525    HOH  1205        DISTANCE =  5.07 ANGSTROMS                       
REMARK 525    HOH  1247        DISTANCE =  9.43 ANGSTROMS                       
REMARK 525    HOH  1263        DISTANCE =  7.02 ANGSTROMS                       
REMARK 525    HOH  1284        DISTANCE =  6.25 ANGSTROMS                       
REMARK 525    HOH  1293        DISTANCE =  5.24 ANGSTROMS                       
REMARK 525    HOH  1303        DISTANCE =  5.52 ANGSTROMS                       
REMARK 525    HOH  1309        DISTANCE =  6.48 ANGSTROMS                       
REMARK 525    HOH  1388        DISTANCE =  9.16 ANGSTROMS                       
REMARK 525    HOH  1389        DISTANCE =  7.79 ANGSTROMS                       
REMARK 900                                                                      
REMARK 900 RELATED ENTRIES                                                      
REMARK 900 RELATED ID: 1C5N   RELATED DB: PDB                                   
REMARK 900 THE SAME PROTEIN COMPLEXED WITH A DIFFERENT INHIBITOR                
DBREF  1O2G L    1H   15  UNP    P00734   THRB_HUMAN     328    363             
DBREF  1O2G H   16   247  UNP    P00734   THRB_HUMAN     364    622             
SEQRES   1 L   36  THR PHE GLY SER GLY GLU ALA ASP CYS GLY LEU ARG PRO          
SEQRES   2 L   36  LEU PHE GLU LYS LYS SER LEU GLU ASP LYS THR GLU ARG          
SEQRES   3 L   36  GLU LEU LEU GLU SER TYR ILE ASP GLY ARG                      
SEQRES   1 H  259  ILE VAL GLU GLY SER ASP ALA GLU ILE GLY MET SER PRO          
SEQRES   2 H  259  TRP GLN VAL MET LEU PHE ARG LYS SER PRO GLN GLU LEU          
SEQRES   3 H  259  LEU CYS GLY ALA SER LEU ILE SER ASP ARG TRP VAL LEU          
SEQRES   4 H  259  THR ALA ALA HIS CYS LEU LEU TYR PRO PRO TRP ASP LYS          
SEQRES   5 H  259  ASN PHE THR GLU ASN ASP LEU LEU VAL ARG ILE GLY LYS          
SEQRES   6 H  259  HIS SER ARG THR ARG TYR GLU ARG ASN ILE GLU LYS ILE          
SEQRES   7 H  259  SER MET LEU GLU LYS ILE TYR ILE HIS PRO ARG TYR ASN          
SEQRES   8 H  259  TRP ARG GLU ASN LEU ASP ARG ASP ILE ALA LEU MET LYS          
SEQRES   9 H  259  LEU LYS LYS PRO VAL ALA PHE SER ASP TYR ILE HIS PRO          
SEQRES  10 H  259  VAL CYS LEU PRO ASP ARG GLU THR ALA ALA SER LEU LEU          
SEQRES  11 H  259  GLN ALA GLY TYR LYS GLY ARG VAL THR GLY TRP GLY ASN          
SEQRES  12 H  259  LEU LYS GLU THR TRP THR ALA ASN VAL GLY LYS GLY GLN          
SEQRES  13 H  259  PRO SER VAL LEU GLN VAL VAL ASN LEU PRO ILE VAL GLU          
SEQRES  14 H  259  ARG PRO VAL CYS LYS ASP SER THR ARG ILE ARG ILE THR          
SEQRES  15 H  259  ASP ASN MET PHE CYS ALA GLY TYR LYS PRO ASP GLU GLY          
SEQRES  16 H  259  LYS ARG GLY ASP ALA CYS GLU GLY ASP SER GLY GLY PRO          
SEQRES  17 H  259  PHE VAL MET LYS SER PRO PHE ASN ASN ARG TRP TYR GLN          
SEQRES  18 H  259  MET GLY ILE VAL SER TRP GLY GLU GLY CYS ASP ARG ASP          
SEQRES  19 H  259  GLY LYS TYR GLY PHE TYR THR HIS VAL PHE ARG LEU LYS          
SEQRES  20 H  259  LYS TRP ILE GLN LYS VAL ILE ASP GLN PHE GLY GLU              
SEQRES   1 I   11  ASP PHE GLU GLU ILE PRO GLU GLU TYS LEU GLN                  
MODRES 1O2G TYS I   63  TYR  SULFONATED TYROSINE                                
HET    TYS  I  63      24                                                       
HET     NA    409       1                                                       
HET    696    246      42                                                       
HETNAM     TYS SULFONATED TYROSINE                                              
HETNAM      NA SODIUM ION                                                       
HETNAM     696 3-{5-[AMINO(IMINIO)METHYL]-1H-INDOL-2-YL}-1,1'-                  
HETNAM   2 696  BIPHENYL-2-OLATE                                                
HETSYN     696 CRA_8696                                                         
FORMUL   3  TYS    C9 H11 N O6 S                                                
FORMUL   4   NA    NA 1+                                                        
FORMUL   5  696    C21 H17 N3 O                                                 
FORMUL   6  HOH   *308(H2 O)                                                    
HELIX    1   1 PHE L    7  SER L   11  5                                   5    
HELIX    2   2 THR L   14B TYR L   14J 1                                   9    
HELIX    3   3 ALA H   55  CYS H   58  5                                   4    
HELIX    4   4 PRO H   60B ASP H   60E 5                                   4    
HELIX    5   5 THR H   60I ASN H   62  5                                   3    
HELIX    6   6 ASP H  125  LEU H  130  1                                   9    
HELIX    7   7 GLU H  164  ASP H  170  1                                   7    
HELIX    8   8 LYS H  185  GLY H  186C 5                                   5    
HELIX    9   9 VAL H  231  GLY H  246  1                                  16    
HELIX   10  10 PRO I   60  LEU I   64  5                                   5    
SHEET    1   A 7 SER H  20  ASP H  21  0                                        
SHEET    2   A 7 GLN H 156  PRO H 161 -1  O  VAL H 157   N  SER H  20           
SHEET    3   A 7 LYS H 135  GLY H 140 -1  N  GLY H 136   O  LEU H 160           
SHEET    4   A 7 PRO H 198  LYS H 202 -1  O  VAL H 200   N  ARG H 137           
SHEET    5   A 7 TRP H 207  TRP H 215 -1  O  TYR H 208   N  MET H 201           
SHEET    6   A 7 GLY H 226  HIS H 230 -1  O  PHE H 227   N  TRP H 215           
SHEET    7   A 7 MET H 180  ALA H 183 -1  N  PHE H 181   O  TYR H 228           
SHEET    1   B 7 LYS H  81  SER H  83  0                                        
SHEET    2   B 7 LEU H  64  ILE H  68 -1  N  ILE H  68   O  LYS H  81           
SHEET    3   B 7 GLN H  30  ARG H  35 -1  N  PHE H  34   O  LEU H  65           
SHEET    4   B 7 GLU H  39  LEU H  46 -1  O  LEU H  41   N  LEU H  33           
SHEET    5   B 7 TRP H  51  THR H  54 -1  O  LEU H  53   N  SER H  45           
SHEET    6   B 7 ALA H 104  LEU H 108 -1  O  MET H 106   N  VAL H  52           
SHEET    7   B 7 LEU H  85  ILE H  90 -1  N  LYS H  87   O  LYS H 107           
SSBOND   1 CYS L    1    CYS H  122                                             
SSBOND   2 CYS H   42    CYS H   58                                             
SSBOND   3 CYS H  168    CYS H  182                                             
SSBOND   4 CYS H  191    CYS H  220                                             
CISPEP   1 SER H   36A   PRO H   37          0       -16.89                     
CRYST1   71.360   72.060   72.960  90.00 101.77  90.00 C 1 2 1       4          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.014013  0.000000  0.002920        0.00000                         
SCALE2      0.000000  0.013877  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.014001        0.00000