PDB entry 1hd4

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HEADER    GLYCOPROTEIN                            07-NOV-00   1HD4              
TITLE     SOLUTION STRUCTURE OF THE A-SUBUNIT OF HUMAN CHORIONIC                
TITLE    2 GONADOTROPIN [MODELED WITH DIANTENNARY GLYCAN AT ASN78]              
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: CHORIONIC GONADOTROPIN;                                    
COMPND   3 CHAIN: A;                                                            
COMPND   4 OTHER_DETAILS: GLYCOSYLATED AT ASN52 AND ASN78                       
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 OTHER_DETAILS: ISOLATED FROM URINE OF PREGNANT WOMAN                 
KEYWDS    HCG, GLYCOPROTEIN, CHORIONIC GONADOTROPIN, GLYCOPROTEIN               
KEYWDS   2 STRUCTURE, NMR, XPLOR                                                
EXPDTA    NMR, 26 STRUCTURES                                                    
AUTHOR    P.J.A.ERBEL,Y.KARIMI-NEJAD,J.A.VAN KUIK,R.BOELENS,                    
AUTHOR   2 J.P.KAMERLING,J.F.G.VLIEGENTHART                                     
REVDAT   2   01-APR-03 1HD4    1       JRNL                                     
REVDAT   1   07-DEC-00 1HD4    0                                                
JRNL        AUTH   P.J.ERBEL,Y.KARIMI-NEJAD,J.A.VAN KUIK,R.BOELENS,             
JRNL        AUTH 2 J.P.KAMERLING,J.F.VLIEGENTHART                               
JRNL        TITL   EFFECTS OF THE N-LINKED GLYCANS ON THE 3D                    
JRNL        TITL 2 STRUCTURE OF THE FREE ALPHA-SUBUNIT OF HUMAN                 
JRNL        TITL 3 CHORIONIC GONADOTROPIN.                                      
JRNL        REF    BIOCHEMISTRY                  V.  39  6012 2000              
JRNL        REFN   ASTM BICHAW  US ISSN 0006-2960                               
REMARK   1                                                                      
REMARK   2                                                                      
REMARK   2 RESOLUTION. NOT APPLICABLE.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : XPLOR 3.851                                          
REMARK   3   AUTHORS     : BRUNGER                                              
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 1HD4 COMPLIES WITH FORMAT V. 3.0, 1-DEC-2006                         
REMARK   4                                                                      
REMARK   4 THIS IS THE REMEDIATED VERSION OF THIS PDB ENTRY.                    
REMARK   4 REMEDIATED DATA FILE REVISION 3.101 (2007-05-31)                     
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY EBI  .                              
REMARK 100 THE EBI ID CODE IS  EBI-5506  .                                      
REMARK 210                                                                      
REMARK 210 EXPERIMENTAL DETAILS                                                 
REMARK 210  EXPERIMENT TYPE                : NMR                                
REMARK 210  TEMPERATURE           (KELVIN) : 328                                
REMARK 210  PH                             : 5.1                                
REMARK 210  IONIC STRENGTH                 : 100 MM                             
REMARK 210  PRESSURE                       : NULL                               
REMARK 210  SAMPLE CONTENTS                : NULL                               
REMARK 210                                                                      
REMARK 210  NMR EXPERIMENTS CONDUCTED      : NULL                               
REMARK 210  SPECTROMETER FIELD STRENGTH    : 500 MHZ, 600 MHZ, 750 MHZ          
REMARK 210  SPECTROMETER MODEL             : AMX, DRX                           
REMARK 210  SPECTROMETER MANUFACTURER      : BRUKER, VARIAN                     
REMARK 210                                                                      
REMARK 210  STRUCTURE DETERMINATION.                                            
REMARK 210   SOFTWARE USED                 : XPLOR                              
REMARK 210   METHOD USED                   : DG/SA                              
REMARK 210                                                                      
REMARK 210 CONFORMERS, NUMBER CALCULATED   : 150                                
REMARK 210 CONFORMERS, NUMBER SUBMITTED    : 26                                 
REMARK 210 CONFORMERS, SELECTION CRITERIA  : NULL                               
REMARK 210                                                                      
REMARK 210 BEST REPRESENTATIVE CONFORMER IN THIS ENSEMBLE : 1                   
REMARK 210                                                                      
REMARK 210 REMARK: STRUCTURAL STATISTICS FOR THE FAMILY OF 27 AHCG              
REMARK 210  CONFORMERS EXCLUDE THE FLEXIBLE LOOP AND ENDS, SEGMENTS 1-10,       
REMARK 210  29-58 AND 85-92 (SEE REFERENCE)                                     
REMARK 215                                                                      
REMARK 215 NMR STUDY                                                            
REMARK 215 THE COORDINATES IN THIS ENTRY WERE GENERATED FROM SOLUTION           
REMARK 215 NMR DATA.  PROTEIN DATA BANK CONVENTIONS REQUIRE THAT                
REMARK 215 CRYST1 AND SCALE RECORDS BE INCLUDED, BUT THE VALUES ON              
REMARK 215 THESE RECORDS ARE MEANINGLESS.                                       
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: ENGH AND HUBER, 1991                                
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500  1 LEU A  26   N   -  CA  -  CB  ANGL. DEV. = -5.3 DEGREES           
REMARK 500  3 GLU A  77   N   -  CA  -  C   ANGL. DEV. = -6.1 DEGREES           
REMARK 500  4 LEU A  26   N   -  CA  -  CB  ANGL. DEV. = -5.3 DEGREES           
REMARK 500  4 GLU A  77   N   -  CA  -  C   ANGL. DEV. = -5.5 DEGREES           
REMARK 500  5 GLU A  77   N   -  CA  -  C   ANGL. DEV. = -5.4 DEGREES           
REMARK 500  6 GLU A  77   N   -  CA  -  C   ANGL. DEV. = -5.5 DEGREES           
REMARK 500  7 GLU A  77   N   -  CA  -  C   ANGL. DEV. = -5.7 DEGREES           
REMARK 500  8 GLU A  77   N   -  CA  -  C   ANGL. DEV. = -5.8 DEGREES           
REMARK 500  9 GLU A  77   N   -  CA  -  C   ANGL. DEV. = -5.9 DEGREES           
REMARK 500 10 LEU A  26   N   -  CA  -  CB  ANGL. DEV. = -5.2 DEGREES           
REMARK 500 12 LEU A  26   CA  -  CB  -  CG  ANGL. DEV. = -5.2 DEGREES           
REMARK 500 12 GLU A  77   N   -  CA  -  C   ANGL. DEV. = -6.3 DEGREES           
REMARK 500 13 GLU A  77   N   -  CA  -  C   ANGL. DEV. = -5.4 DEGREES           
REMARK 500 15 GLU A  77   N   -  CA  -  C   ANGL. DEV. = -5.5 DEGREES           
REMARK 500 16 GLU A  77   N   -  CA  -  C   ANGL. DEV. = -5.9 DEGREES           
REMARK 500 18 LEU A  26   N   -  CA  -  CB  ANGL. DEV. = -6.2 DEGREES           
REMARK 500 20 GLU A  77   N   -  CA  -  C   ANGL. DEV. = -5.5 DEGREES           
REMARK 500 22 GLU A  77   N   -  CA  -  C   ANGL. DEV. = -5.7 DEGREES           
REMARK 500 23 GLU A  77   N   -  CA  -  C   ANGL. DEV. = -5.9 DEGREES           
REMARK 500 24 LEU A  26   N   -  CA  -  CB  ANGL. DEV. = -5.6 DEGREES           
REMARK 500 24 LEU A  26   CA  -  CB  -  CG  ANGL. DEV. = -5.4 DEGREES           
REMARK 500 24 GLU A  77   N   -  CA  -  C   ANGL. DEV. = -5.5 DEGREES           
REMARK 500 25 LEU A  26   N   -  CA  -  CB  ANGL. DEV. = -5.2 DEGREES           
REMARK 500 25 GLU A  77   N   -  CA  -  C   ANGL. DEV. = -5.5 DEGREES           
REMARK 500 26 GLU A  77   N   -  CA  -  C   ANGL. DEV. = -5.3 DEGREES           
DBREF  1HD4 A    1    92  UNP    P01215   GLHA_HUMAN      25    116             
SEQRES   1 A   92  ALA PRO ASP VAL GLN ASP CYS PRO GLU CYS THR LEU GLN          
SEQRES   2 A   92  GLU ASN PRO PHE PHE SER GLN PRO GLY ALA PRO ILE LEU          
SEQRES   3 A   92  GLN CYS MET GLY CYS CYS PHE SER ARG ALA TYR PRO THR          
SEQRES   4 A   92  PRO LEU ARG SER LYS LYS THR MET LEU VAL GLN LYS ASN          
SEQRES   5 A   92  VAL THR SER GLU SER THR CYS CYS VAL ALA LYS SER TYR          
SEQRES   6 A   92  ASN ARG VAL THR VAL MET GLY GLY PHE LYS VAL GLU ASN          
SEQRES   7 A   92  HIS THR ALA CYS HIS CYS SER THR CYS TYR TYR HIS LYS          
SEQRES   8 A   92  SER                                                          
MODRES 1HD4 ASN A   78  ASN  GLYCOSYLATION SITE                                 
HET    NAG  A 178      27                                                       
HET    NAG  A 179      27                                                       
HET    BMA  A 180      20                                                       
HET    MAN  A 181      21                                                       
HET    MAN  A 182      21                                                       
HET    NAG  A 183      27                                                       
HET    NAG  A 184      27                                                       
HET    GAL  A 185      22                                                       
HET    GAL  A 186      22                                                       
HETNAM     NAG N-ACETYL-D-GLUCOSAMINE                                           
HETNAM     BMA BETA-D-MANNOSE                                                   
HETNAM     MAN ALPHA-D-MANNOSE                                                  
HETNAM     GAL BETA-D-GALACTOSE                                                 
HETSYN     NAG NAG                                                              
FORMUL   2  NAG    4(C8 H15 N O6)                                               
FORMUL   2  BMA    C6 H12 O6                                                    
FORMUL   2  MAN    2(C6 H12 O6)                                                 
FORMUL   2  GAL    2(C6 H12 O6)                                                 
SSBOND   1 CYS A    7    CYS A   31                                             
SSBOND   2 CYS A   10    CYS A   60                                             
SSBOND   3 CYS A   28    CYS A   82                                             
SSBOND   4 CYS A   32    CYS A   84                                             
SSBOND   5 CYS A   59    CYS A   87                                             
LINK         ND2 ASN A  78                 C1  NAG A 178                        
LINK         O4  NAG A 178                 C1  NAG A 179                        
LINK         O4  NAG A 179                 C1  BMA A 180                        
LINK         O6  BMA A 180                 C1  MAN A 182                        
LINK         O3  BMA A 180                 C1  MAN A 181                        
LINK         O2  MAN A 181                 C1  NAG A 184                        
LINK         O2  MAN A 182                 C1  NAG A 183                        
LINK         O4  NAG A 183                 C1  GAL A 186                        
LINK         O4  NAG A 184                 C1  GAL A 185                        
CRYST1    1.000    1.000    1.000  90.00  90.00  90.00 P 1           1          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      1.000000  0.000000  0.000000        0.00000                         
SCALE2      0.000000  1.000000  0.000000        0.00000                         
SCALE3      0.000000  0.000000  1.000000        0.00000                         
MODEL        1