PDB entry 1h8d

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HEADER    SERINE PROTEASE                         01-FEB-01   1H8D              
TITLE     X-RAY STRUCTURE OF THE HUMAN ALPHA-THROMBIN COMPLEX WITH A            
TITLE    2 TRIPEPTIDE PHOSPHONATE INHIBITOR.                                    
COMPND    MOL_ID: 1;                                                            
COMPND   2 MOLECULE: THROMBIN;                                                  
COMPND   3 CHAIN: L;                                                            
COMPND   4 FRAGMENT: THROMBIN LIGHT CHAIN;                                      
COMPND   5 SYNONYM: COAGULATION FACTOR II;                                      
COMPND   6 EC: 3.4.21.5;                                                        
COMPND   7 MOL_ID: 2;                                                           
COMPND   8 MOLECULE: THROMBIN;                                                  
COMPND   9 CHAIN: H;                                                            
COMPND  10 FRAGMENT: THROMBIN HEAVY CHAIN;                                      
COMPND  11 SYNONYM: COAGULATION FACTOR II;                                      
COMPND  12 EC: 3.4.21.5;                                                        
COMPND  13 MOL_ID: 3;                                                           
COMPND  14 MOLECULE: HIRUDIN I;                                                 
COMPND  15 CHAIN: I;                                                            
COMPND  16 FRAGMENT: RESIDUES 55 TO 64;                                         
COMPND  17 SYNONYM: LEPIRUDIN                                                   
SOURCE    MOL_ID: 1;                                                            
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   3 ORGANISM_COMMON: HUMAN;                                              
SOURCE   4 MOL_ID: 2;                                                           
SOURCE   5 ORGANISM_SCIENTIFIC: HOMO SAPIENS;                                   
SOURCE   6 ORGANISM_COMMON: HUMAN;                                              
SOURCE   7 MOL_ID: 3;                                                           
SOURCE   8 ORGANISM_SCIENTIFIC: HIRUDO MEDICINALIS;                             
SOURCE   9 ORGANISM_COMMON: MEDICINAL LEECH                                     
KEYWDS    SERINE PROTEASE                                                       
EXPDTA    X-RAY DIFFRACTION                                                     
AUTHOR    E.SKORDALAKES,G.G.DODSON,D.GREEN,J.DEADMAN                            
REVDAT   2   16-AUG-01 1H8D    1       REVDAT JRNL   REMARK SSBOND              
REVDAT   2 2                   1       LINK   HETATM CONECT MASTER              
REVDAT   1   26-FEB-01 1H8D    0                                                
JRNL        AUTH   E.SKORDALAKES,G.G.DODSON,D.S.GREEN,C.A.GOODWIN,              
JRNL        AUTH 2 M.F.SCULLY,H.R.HUDSON,V.V.KAKKAR,J.J.DEADMAN                 
JRNL        TITL   INHIBITION OF HUMAN ALPHA-THROMBIN BY A                      
JRNL        TITL 2 PHOSPHONATE TRIPEPTIDE PROCEEDS VIA A METASTABLE             
JRNL        TITL 3 PENTACOORDINATED PHOSPHORUS INTERMEDIATE.                    
JRNL        REF    J.MOL.BIOL.                   V. 311   549 2001              
JRNL        REFN   ASTM JMOBAK  UK ISSN 0022-2836                               
REMARK   1                                                                      
REMARK   1 REFERENCE 1                                                          
REMARK   1  AUTH   J.A.BERTRAND,J.OLEKSYSZYN,C.M.KAM,B.BODUSZEK,                
REMARK   1  AUTH 2 S.PRESNELL,R.R.PLASKON,F.L.SUDDATH,J.C.POWERS,               
REMARK   1  AUTH 3 L.D.WILLIAMS                                                 
REMARK   1  TITL   INHIBITION OF TRYPSIN AND THROMBIN BY AMINO                  
REMARK   1  TITL 2 (4-AMIDINOPHENYL)METHANEPHOSPHONATE DIPHENYL ESTER           
REMARK   1  TITL 3 DERIVATIVES: X-RAY AND MOLECULAR MODELS                      
REMARK   1  REF    BIOCHEMISTRY                  V.  35  3147 1996              
REMARK   1  REFN   ASTM BICHAW  US ISSN 0006-2960                               
REMARK   2                                                                      
REMARK   2 RESOLUTION. 1.40 ANGSTROMS.                                          
REMARK   3                                                                      
REMARK   3 REFINEMENT.                                                          
REMARK   3   PROGRAM     : REFMAC                                               
REMARK   3   AUTHORS     : MURSHUDOV,VAGIN,DODSON                               
REMARK   3                                                                      
REMARK   3  DATA USED IN REFINEMENT.                                            
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.40                           
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 14.00                          
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 0.000                          
REMARK   3   COMPLETENESS FOR RANGE        (%) : 95.4                           
REMARK   3   NUMBER OF REFLECTIONS             : 68615                          
REMARK   3                                                                      
REMARK   3  FIT TO DATA USED IN REFINEMENT.                                     
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT                      
REMARK   3   FREE R VALUE TEST SET SELECTION  : NULL                            
REMARK   3   R VALUE     (WORKING + TEST SET) : NULL                            
REMARK   3   R VALUE            (WORKING SET) : 0.170                           
REMARK   3   FREE R VALUE                     : 0.220                           
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : NULL                            
REMARK   3   FREE R VALUE TEST SET COUNT      : NULL                            
REMARK   3                                                                      
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.                    
REMARK   3   PROTEIN ATOMS            : 2346                                    
REMARK   3   NUCLEIC ACID ATOMS       : 0                                       
REMARK   3   HETEROGEN ATOMS          : 57                                      
REMARK   3   SOLVENT ATOMS            : NULL                                    
REMARK   3                                                                      
REMARK   3  B VALUES.                                                           
REMARK   3   FROM WILSON PLOT           (A**2) : 16.27                          
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 18.50                          
REMARK   3   OVERALL ANISOTROPIC B VALUE.                                       
REMARK   3    B11 (A**2) : NULL                                                 
REMARK   3    B22 (A**2) : NULL                                                 
REMARK   3    B33 (A**2) : NULL                                                 
REMARK   3    B12 (A**2) : NULL                                                 
REMARK   3    B13 (A**2) : NULL                                                 
REMARK   3    B23 (A**2) : NULL                                                 
REMARK   3                                                                      
REMARK   3  ESTIMATED OVERALL COORDINATE ERROR.                                 
REMARK   3   ESU BASED ON R VALUE                            (A): NULL          
REMARK   3   ESU BASED ON FREE R VALUE                       (A): NULL          
REMARK   3   ESU BASED ON MAXIMUM LIKELIHOOD                 (A): NULL          
REMARK   3   ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): NULL          
REMARK   3                                                                      
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES.                                   
REMARK   3   DISTANCE RESTRAINTS.                    RMS    SIGMA               
REMARK   3    BOND LENGTH                     (A) : 0.008 ; NULL                
REMARK   3    ANGLE DISTANCE                  (A) : 1.200 ; NULL                
REMARK   3    INTRAPLANAR 1-4 DISTANCE        (A) : NULL  ; NULL                
REMARK   3    H-BOND OR METAL COORDINATION    (A) : NULL  ; NULL                
REMARK   3                                                                      
REMARK   3   PLANE RESTRAINT                  (A) : NULL  ; NULL                
REMARK   3   CHIRAL-CENTER RESTRAINT       (A**3) : NULL  ; NULL                
REMARK   3                                                                      
REMARK   3   NON-BONDED CONTACT RESTRAINTS.                                     
REMARK   3    SINGLE TORSION                  (A) : NULL  ; NULL                
REMARK   3    MULTIPLE TORSION                (A) : NULL  ; NULL                
REMARK   3    H-BOND (X...Y)                  (A) : NULL  ; NULL                
REMARK   3    H-BOND (X-H...Y)                (A) : NULL  ; NULL                
REMARK   3                                                                      
REMARK   3   CONFORMATIONAL TORSION ANGLE RESTRAINTS.                           
REMARK   3    SPECIFIED                 (DEGREES) : NULL  ; NULL                
REMARK   3    PLANAR                    (DEGREES) : NULL  ; NULL                
REMARK   3    STAGGERED                 (DEGREES) : NULL  ; NULL                
REMARK   3    TRANSVERSE                (DEGREES) : NULL  ; NULL                
REMARK   3                                                                      
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.    RMS    SIGMA                
REMARK   3   MAIN-CHAIN BOND              (A**2) : NULL  ; NULL                 
REMARK   3   MAIN-CHAIN ANGLE             (A**2) : NULL  ; NULL                 
REMARK   3   SIDE-CHAIN BOND              (A**2) : NULL  ; NULL                 
REMARK   3   SIDE-CHAIN ANGLE             (A**2) : NULL  ; NULL                 
REMARK   3                                                                      
REMARK   3  OTHER REFINEMENT REMARKS: NULL                                      
REMARK   4                                                                      
REMARK   4 1H8D COMPLIES WITH FORMAT V. 3.0, 1-DEC-2006                         
REMARK   4                                                                      
REMARK   4 THIS IS THE REMEDIATED VERSION OF THIS PDB ENTRY.                    
REMARK   4 REMEDIATED DATA FILE REVISION 3.101 (2007-05-31)                     
REMARK 100                                                                      
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY EBI.                                
REMARK 100 THE EBI ID CODE IS  EBI-5851.                                        
REMARK 200                                                                      
REMARK 200 EXPERIMENTAL DETAILS                                                 
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION                  
REMARK 200  DATE OF DATA COLLECTION        : 07-JUN-1998                        
REMARK 200  TEMPERATURE           (KELVIN) : 100.0                              
REMARK 200  PH                             : 7.20                               
REMARK 200  NUMBER OF CRYSTALS USED        : 1                                  
REMARK 200                                                                      
REMARK 200  SYNCHROTRON              (Y/N) : Y                                  
REMARK 200  RADIATION SOURCE               : SRS                                
REMARK 200  BEAMLINE                       : PX7.2                              
REMARK 200  X-RAY GENERATOR MODEL          : NULL                               
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M                                  
REMARK 200  WAVELENGTH OR RANGE        (A) : 1.48800                            
REMARK 200  MONOCHROMATOR                  : NULL                               
REMARK 200  OPTICS                         : NULL                               
REMARK 200                                                                      
REMARK 200  DETECTOR TYPE                  : NULL                               
REMARK 200  DETECTOR MANUFACTURER          : NULL                               
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : DENZO                              
REMARK 200  DATA SCALING SOFTWARE          : SCALEPACK                          
REMARK 200                                                                      
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 68615                              
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.400                              
REMARK 200  RESOLUTION RANGE LOW       (A) : 15.000                             
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : 5.000                              
REMARK 200                                                                      
REMARK 200 OVERALL.                                                             
REMARK 200  COMPLETENESS FOR RANGE     (%) : 95.4                               
REMARK 200  DATA REDUNDANCY                : 3.000                              
REMARK 200  R MERGE                    (I) : 0.05200                            
REMARK 200  R SYM                      (I) : 0.05600                            
REMARK 200   FOR THE DATA SET  : 23.0000                            
REMARK 200                                                                      
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.                                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.40                     
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.45                     
REMARK 200  COMPLETENESS FOR SHELL     (%) : 96.6                               
REMARK 200  DATA REDUNDANCY IN SHELL       : 2.00                               
REMARK 200  R MERGE FOR SHELL          (I) : 0.14200                            
REMARK 200  R SYM FOR SHELL            (I) : 0.13100                            
REMARK 200   FOR SHELL         : 6.000                              
REMARK 200                                                                      
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH                              
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMEN         
REMARK 200 SOFTWARE USED: AMORE                                                 
REMARK 200 STARTING MODEL: 1HGT                                                 
REMARK 200                                                                      
REMARK 200 REMARK: NULL                                                         
REMARK 280                                                                      
REMARK 280 CRYSTAL                                                              
REMARK 280 SOLVENT CONTENT, VS   (%): 54.00                                     
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.59                     
REMARK 280                                                                      
REMARK 280 CRYSTALLIZATION CONDITIONS: PH 7.2                                   
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY                                            
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: C 1 2 1                          
REMARK 290                                                                      
REMARK 290      SYMOP   SYMMETRY                                                
REMARK 290     NNNMMM   OPERATOR                                                
REMARK 290       1555   X,Y,Z                                                   
REMARK 290       2555   -X,Y,-Z                                                 
REMARK 290       3555   1/2+X,1/2+Y,Z                                           
REMARK 290       4555   1/2-X,1/2+Y,-Z                                          
REMARK 290                                                                      
REMARK 290     WHERE NNN -> OPERATOR NUMBER                                     
REMARK 290           MMM -> TRANSLATION VECTOR                                  
REMARK 290                                                                      
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS                            
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM             
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY                
REMARK 290 RELATED MOLECULES.                                                   
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   2 -1.000000  0.000000  0.000000        0.00000            
REMARK 290   SMTRY2   2  0.000000  1.000000  0.000000        0.00000            
REMARK 290   SMTRY3   2  0.000000  0.000000 -1.000000        0.00000            
REMARK 290   SMTRY1   3  1.000000  0.000000  0.000000       35.32000            
REMARK 290   SMTRY2   3  0.000000  1.000000  0.000000       35.80000            
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000        0.00000            
REMARK 290   SMTRY1   4 -1.000000  0.000000  0.000000       35.32000            
REMARK 290   SMTRY2   4  0.000000  1.000000  0.000000       35.80000            
REMARK 290   SMTRY3   4  0.000000  0.000000 -1.000000        0.00000            
REMARK 290                                                                      
REMARK 290 REMARK: NULL                                                         
REMARK 300                                                                      
REMARK 300 BIOMOLECULE: 1                                                       
REMARK 300 THIS ENTRY CONTAINS THE CRYSTALLOGRAPHIC ASYMMETRIC UNIT             
REMARK 300 WHICH CONSISTS OF 3 CHAIN(S). SEE REMARK 350 FOR                     
REMARK 300 INFORMATION ON GENERATING THE BIOLOGICAL MOLECULE(S).                
REMARK 350                                                                      
REMARK 350 GENERATING THE BIOMOLECULE                                           
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN           
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE                
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS          
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND                          
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.                               
REMARK 350                                                                      
REMARK 350 BIOMOLECULE: 1                                                       
REMARK 350 APPLY THE FOLLOWING TO CHAINS: L, H, I, K, O, P, R, S                
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000            
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000            
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000            
REMARK 375                                                                      
REMARK 375 SPECIAL POSITION                                                     
REMARK 375 THE FOLLOWING ATOMS ARE FOUND TO BE WITHIN 0.15 ANGSTROMS            
REMARK 375 OF A SYMMETRY RELATED ATOM AND ARE ASSUMED TO BE ON SPECIAL          
REMARK 375 POSITIONS.                                                           
REMARK 375                                                                      
REMARK 375 ATOM RES CSSEQI                                                      
REMARK 375      HOH S  55   LIES ON A SPECIAL POSITION.                         
REMARK 375      HOH S  57   LIES ON A SPECIAL POSITION.                         
REMARK 375      HOH S 258   LIES ON A SPECIAL POSITION.                         
REMARK 375      HOH S 269   LIES ON A SPECIAL POSITION.                         
REMARK 375      HOH S 531   LIES ON A SPECIAL POSITION.                         
REMARK 470                                                                      
REMARK 470 MISSING ATOM                                                         
REMARK 470 THE FOLLOWING RESIDUES HAVE MISSING ATOMS(M=MODEL NUMBER;            
REMARK 470 RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE NUMBER;          
REMARK 470 I=INSERTION CODE):                                                   
REMARK 470   M RES CSSEQI  ATOMS                                                
REMARK 470     GLU L   1A   CG    CD    OE1   OE2                               
REMARK 470     ASP L   1C   OD1   OD2                                           
REMARK 470     CYS L   1    N                                                   
REMARK 470     SER L  14L   N     CA    C     O     CB                          
REMARK 470     ARG H  75    CD    NE    CZ    NH1   NH2                         
REMARK 470     LYS H 236    CD    CE    NZ                                      
REMARK 470     CYS H 259    N     CA    C     O     CB                          
REMARK 470     SER H 261    N     CA    C     O     CB                          
REMARK 470     SER H 262    N     CA    C     O     CB                          
REMARK 470     VAL H 263    N     CA    C     O     CB    CG1                   
REMARK 470     LEU H 264    N     CA    C     O     CB    CG    CD1             
REMARK 470     ILE H 265    N     CA    C     O     CB    CG1   CG2             
REMARK 470     VAL H 266    N     CA    C     O     CB    CG1                   
REMARK 470     VAL H 267    N     CA    C     O     CB    CG1                   
REMARK 470     CYS H 268    N     CA    C     O     CB                          
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT                     
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT.                            
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI                             
REMARK 500   CG1  ILE H    90     CD1  ILE H   265              0.71            
REMARK 500   CG   LEU H    85     CD2  LEU H   264              0.80            
REMARK 500   CD1  ILE H    90     CD1  ILE H   265              1.16            
REMARK 500   NZ   LYS L     9     O    HOH O    42              1.24            
REMARK 500   CB   SER L    11     OG   SER L    14L             1.48            
REMARK 500   CB   SER H    27     OG   SER H   261              1.48            
REMARK 500   CB   VAL H    66     CG2  VAL H   263              1.48            
REMARK 500   CB   SER H    72     OG   SER H   262              1.49            
REMARK 500   CB   VAL H   138     CG2  VAL H   266              1.49            
REMARK 500   CB   VAL H   157     CG2  VAL H   267              1.49            
REMARK 500   CB   LEU H    85     CD2  LEU H   264              1.50            
REMARK 500   CB   ILE H    90     CD1  ILE H   265              1.50            
REMARK 500   O    ILE L    14K    O    HOH O    66              1.54            
REMARK 500   CD1  LEU H    85     CD2  LEU H   264              1.72            
REMARK 500   CB   CYS H    42     SG   CYS H   259              1.73            
REMARK 500   SD   MET H   180     O    HOH S   324              1.75            
REMARK 500   C54  PHW K     1     O    HOH S   504              1.79            
REMARK 500   C52  PHW K     1     O    HOH P     6              1.80            
REMARK 500   O    HOH O    45     O    HOH O    47              1.82            
REMARK 500   O    HOH S    30     O    HOH S   109              1.83            
REMARK 500   OE1  GLU L    13     O    HOH O    54              1.88            
REMARK 500   O    HOH O    24     O    HOH O    43              1.88            
REMARK 500   O    HOH R    31     O    HOH R    32              1.88            
REMARK 500   SD   MET H    84     O    HOH R    38              1.94            
REMARK 500   CG1  VAL H    66     CG2  VAL H   263              1.97            
REMARK 500   O    HOH O    11     O    HOH O    12              1.97            
REMARK 500   O    HOH S   231     O    HOH S   503              1.99            
REMARK 500   O    HOH S     1     O    HOH S    27              2.00            
REMARK 500   O    HOH S    37     O    HOH S   142              2.00            
REMARK 500   N    THR H   177     CE   MET H   180              2.01            
REMARK 500   O    HOH S   152     O    HOH S   153              2.02            
REMARK 500   O    HOH S   203     O    HOH S   220              2.02            
REMARK 500   NZ   LYS H    87     O    HOH S   290              2.03            
REMARK 500   O    HOH S    12     O    HOH S    83              2.04            
REMARK 500   O    HOH S    28     O    HOH S   103              2.04            
REMARK 500   CG   LYS H   236     O    HOH S    67              2.05            
REMARK 500   O    LEU I    10     O    HOH R    39              2.05            
REMARK 500   O    ILE L    14K    O    HOH O    59              2.06            
REMARK 500   O    HOH S    25     O    HOH S   301              2.06            
REMARK 500   O    HOH S   140     O    HOH S   157              2.06            
REMARK 500   O    HOH S   482     O    HOH S   483              2.06            
REMARK 500   O    HOH S    16     O    HOH S   169              2.08            
REMARK 500   O    HOH S   247     O    HOH S   550              2.08            
REMARK 500   CG   ARG H    50     O    HOH S   185              2.09            
REMARK 500   NE   ARG H    97     O    HOH S   317              2.09            
REMARK 500   OE1  GLN H   151     O    HOH S   396              2.09            
REMARK 500   CG   GLU I     4     O    HOH R    28              2.09            
REMARK 500   C53  PHW K     1     O    HOH P     6              2.09            
REMARK 500   O    HOH S   243     O    HOH S   539              2.09            
REMARK 500   O    HOH S    89     O    HOH S   329              2.10            
REMARK 500   O    HOH S   369     O    HOH S   406              2.10            
REMARK 500   O    HOH S   126     O    HOH S   347              2.11            
REMARK 500   OE2  GLU H   127     O    HOH S   364              2.12            
REMARK 500   O    HOH S   135     O    HOH S   365              2.12            
REMARK 500   O    HOH S   326     O    HOH S   329              2.12            
REMARK 500   O    HOH O    23     O    HOH S   122              2.13            
REMARK 500   O    HOH O    34     O    HOH S   404              2.13            
REMARK 500   O    HOH S    57     O    HOH S   277              2.13            
REMARK 500   O    HOH S   138     O    HOH S   227              2.13            
REMARK 500   O    HOH S   194     O    HOH S   456              2.13            
REMARK 500   O    HOH S    60     O    HOH S   125              2.14            
REMARK 500   O    HOH S    89     O    HOH S   304              2.14            
REMARK 500   CE   LYS H    87     O    HOH S   290              2.15            
REMARK 500   OE1  GLN H   244     O    HOH S   551              2.15            
REMARK 500   O    HOH R    14     O    HOH R    15              2.15            
REMARK 500   O    HOH S    41     O    HOH S   381              2.15            
REMARK 500   O    HOH S   363     O    HOH S   365              2.15            
REMARK 500   OG   SER L    11     OG   SER L    14L             2.16            
REMARK 500   CD   ARG H   165     O    HOH S   415              2.16            
REMARK 500   OD1  ASP H   243     O    HOH S   548              2.16            
REMARK 500   O    HOH S   183     O    HOH S   184              2.16            
REMARK 500   O    HOH S   193     O    HOH S   459              2.17            
REMARK 500   O    HOH S   260     O    HOH S   264              2.17            
REMARK 500   O    HOH S   411     O    HOH S   438              2.17            
REMARK 500   O    HOH S   214     O    HOH S   222              2.18            
REMARK 500   O    HOH S   365     O    HOH S   367              2.18            
REMARK 500   C    ILE L    14K    O    HOH O    59              2.19            
REMARK 500   O    HOH O    56     O    HOH O    65              2.19            
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: CLOSE CONTACTS                                             
REMARK 500                                                                      
REMARK 500 THE FOLLOWING ATOMS THAT ARE RELATED BY CRYSTALLOGRAPHIC             
REMARK 500 SYMMETRY ARE IN CLOSE CONTACT.  AN ATOM LOCATED WITHIN 0.15          
REMARK 500 ANGSTROMS OF A SYMMETRY RELATED ATOM IS ASSUMED TO BE ON A           
REMARK 500 SPECIAL POSITION AND IS, THEREFORE, LISTED IN REMARK 375             
REMARK 500 INSTEAD OF REMARK 500.  ATOMS WITH NON-BLANK ALTERNATE               
REMARK 500 LOCATION INDICATORS ARE NOT INCLUDED IN THE CALCULATIONS.            
REMARK 500                                                                      
REMARK 500 DISTANCE CUTOFF:                                                     
REMARK 500 2.2 ANGSTROMS FOR CONTACTS NOT INVOLVING HYDROGEN ATOMS              
REMARK 500 1.6 ANGSTROMS FOR CONTACTS INVOLVING HYDROGEN ATOMS                  
REMARK 500                                                                      
REMARK 500  ATM1  RES C  SSEQI   ATM2  RES C  SSEQI  SSYMOP   DISTANCE          
REMARK 500   O    HOH S   459     O    HOH S   459     2556     1.46            
REMARK 500   O    HOH S   207     O    HOH S   347     3545     1.84            
REMARK 500   O    HOH O    15     O    HOH S    14     3455     1.85            
REMARK 500   O    HOH S    56     O    HOH S    56     2555     2.01            
REMARK 500   O    HOH S     4     O    HOH S   169     2656     2.04            
REMARK 500   O    HOH O    25     O    HOH S   220     3455     2.12            
REMARK 500   O    HOH S   363     O    HOH S   520     4556     2.17            
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND LENGTHS                                      
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,2(A3,1X,A1,I4,A1,1X,A4,3X),F6.3)                  
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: ENGH AND HUBER, 1991                                
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   RES CSSEQI ATM2   DEVIATION                     
REMARK 500    CYS L   1   CA    CYS L   1   CB     0.547                        
REMARK 500    CYS L   1   CA    CYS L   1   C      1.155                        
REMARK 500                                                                      
REMARK 500 GEOMETRY AND STEREOCHEMISTRY                                         
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES                                       
REMARK 500                                                                      
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES              
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE               
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN               
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).                 
REMARK 500                                                                      
REMARK 500 STANDARD TABLE:                                                      
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)              
REMARK 500                                                                      
REMARK 500 EXPECTED VALUES: ENGH AND HUBER, 1991                                
REMARK 500                                                                      
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3                                     
REMARK 500    CYS L   1   CB  -  CA  -  C   ANGL. DEV. =-49.2 DEGREES           
REMARK 500    CYS L   1   CA  -  CB  -  SG  ANGL. DEV. = 22.1 DEGREES           
REMARK 500    CYS H  42   CA  -  CB  -  SG  ANGL. DEV. = 18.0 DEGREES           
REMARK 525                                                                      
REMARK 525 SOLVENT                                                              
REMARK 525 THE FOLLOWING SOLVENT MOLECULES LIE FARTHER THAN EXPECTED            
REMARK 525 FROM THE PROTEIN OR NUCLEIC ACID MOLECULE AND MAY BE                 
REMARK 525 ASSOCIATED WITH A SYMMETRY RELATED MOLECULE (M=MODEL                 
REMARK 525 NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER; SSEQ=SEQUENCE          
REMARK 525 NUMBER; I=INSERTION CODE):                                           
REMARK 525                                                                      
REMARK 525  M RES CSSEQI                                                        
REMARK 525    HOH S   2        DISTANCE =  6.70 ANGSTROMS                       
REMARK 525    HOH S   4        DISTANCE = 10.43 ANGSTROMS                       
REMARK 525    HOH R  20        DISTANCE =  5.35 ANGSTROMS                       
REMARK 525    HOH S  28        DISTANCE =  6.08 ANGSTROMS                       
REMARK 800                                                                      
REMARK 800 SITE                                                                 
REMARK 800 SITE_IDENTIFIER: AC1                                                 
REMARK 800 SITE_DESCRIPTION: TRIPEPTIDE PHOSPHONATE INHIBITOR BINDING           
DBREF  1H8D L    1    14L UNP    P00734   THRB_HUMAN     333    361             
DBREF  1H8D H   16   259  UNP    P00734   THRB_HUMAN     364    622             
DBREF  1H8D I    1    10  UNP    P01050   ITH1_HIRME      55     64             
SEQADV 1H8D TYS I    9  UNP  P01050    TYR    63 MODIFIED RESIDUE               
SEQRES   1 L   29  GLU ALA ASP CYS GLY LEU ARG PRO LEU PHE GLU LYS LYS          
SEQRES   2 L   29  SER LEU GLU ASP LYS THR GLU ARG GLU LEU LEU GLU SER          
SEQRES   3 L   29  TYR ILE SER                                                  
SEQRES   1 H  260  ILE VAL GLU GLY SER ASP ALA GLU ILE GLY MET SER PRO          
SEQRES   2 H  260  TRP GLN VAL MET LEU PHE ARG LYS SER PRO GLN GLU LEU          
SEQRES   3 H  260  LEU CYS GLY ALA SER LEU ILE SER ASP ARG TRP VAL LEU          
SEQRES   4 H  260  THR ALA ALA HIS CYS LEU LEU TYR PRO PRO TRP ASP LYS          
SEQRES   5 H  260  ASN PHE THR GLU ASN ASP LEU LEU VAL ARG ILE GLY LYS          
SEQRES   6 H  260  HIS SER ARG THR ARG TYR GLU ARG ASN ILE GLU LYS ILE          
SEQRES   7 H  260  SER MET LEU GLU LYS ILE TYR ILE HIS PRO ARG TYR ASN          
SEQRES   8 H  260  TRP ARG GLU ASN LEU ASP ARG ASP ILE ALA LEU MET LYS          
SEQRES   9 H  260  LEU LYS LYS PRO VAL ALA PHE SER ASP TYR ILE HIS PRO          
SEQRES  10 H  260  VAL CYS LEU PRO ASP ARG GLU THR ALA ALA SER LEU LEU          
SEQRES  11 H  260  GLN ALA GLY TYR LYS GLY ARG VAL THR GLY TRP GLY ASN          
SEQRES  12 H  260  LEU LYS GLU THR GLY GLN PRO SER VAL LEU GLN VAL VAL          
SEQRES  13 H  260  ASN LEU PRO ILE VAL GLU ARG PRO VAL CYS LYS ASP SER          
SEQRES  14 H  260  THR ARG ILE ARG ILE THR ASP ASN MET PHE CYS ALA GLY          
SEQRES  15 H  260  TYR LYS PRO ASP GLU GLY LYS ARG GLY ASP ALA CYS GLU          
SEQRES  16 H  260  GLY ASP SER GLY GLY PRO PHE VAL MET LYS SER PRO PHE          
SEQRES  17 H  260  ASN ASN ARG TRP TYR GLN MET GLY ILE VAL SER TRP GLY          
SEQRES  18 H  260  GLU GLY CYS ASP ARG ASP GLY LYS TYR GLY PHE TYR THR          
SEQRES  19 H  260  HIS VAL PHE ARG LEU LYS LYS TRP ILE GLN LYS VAL ILE          
SEQRES  20 H  260  ASP GLN PHE GLY CYS SER SER VAL LEU ILE VAL VAL CYS          
SEQRES   1 I   10  ASP PHE GLU GLU ILE PRO GLU GLU TYS LEU                      
MODRES 1H8D TYS I    9  TYR  SULFONATED TYROSINE                                
HET    TYS  I   9      16                                                       
HET    PHW  K   1      53                                                       
HETNAM     TYS SULFONATED TYROSINE                                              
HETNAM     PHW BETA-PHENYL-N-{[(PHENYLMETHYL)OXY]CARBONYL}-D-                   
HETNAM   2 PHW  PHENYLALANYL-N- [(1S,3E)-1-[DIHYDROXY(METHYLOXY)                
HETNAM   3 PHW  (PHENYLOXY)-LAMBDA5-PHOSPHANYL]-4- (METHYLOXY)BUT-3-            
HETNAM   4 PHW  ENYL]-D-PROLINAMIDE                                             
HETSYN     PHW PHENYLMETHYL (1R)-2-[(2R)-2-({[(1S,3E)-1-                        
HETSYN   2 PHW  [DIHYDROXY(METHYLOXY)(PHENYLOXY)- LAMBDA5-PHOSPHANYL]-          
HETSYN   3 PHW  4-(METHYLOXY)BUT-3-ENYL]AMINO}CARBONYL)PYRROLIDIN-1-            
HETSYN   4 PHW  YL]- 1-(DIPHENYLMETHYL)-2-OXOETHYLCARBAMATE                     
FORMUL   3  TYS    C9 H11 N O6 S                                                
FORMUL   4  PHW    C40 H46 N3 O9 P                                              
FORMUL   5  HOH   *684(H2 O)                                                    
HELIX    1   9 PHE L    7  SER L   11  5                                   5    
HELIX    2  10 THR L   14B TYR L   14J 1                                   9    
HELIX    3   1 ALA H   55  CYS H   58  5                                   4    
HELIX    4   2 PRO H   60B ASP H   60E 5                                   4    
HELIX    5   3 THR H   60I ASN H   62  5                                   3    
HELIX    6   4 ASP H  125  LEU H  130  1                                   9    
HELIX    7   5 GLU H  164  SER H  171  1                                   8    
HELIX    8   6 LYS H  185  GLY H  186C 5                                   5    
HELIX    9   7 LEU H  234  GLY H  246  1                                  13    
HELIX   10   8 PRO I    6  LEU I   10  5                                   5    
SHEET    1  HA 7 SER H  20  ASP H  21  0                                        
SHEET    2  HA 7 GLN H 156  PRO H 161 -1  O  VAL H 157   N  SER H  20           
SHEET    3  HA 7 LYS H 135  GLY H 140 -1  O  GLY H 136   N  LEU H 160           
SHEET    4  HA 7 PRO H 198  LYS H 202 -1  O  PRO H 198   N  THR H 139           
SHEET    5  HA 7 TRP H 207  TRP H 215 -1  O  TYR H 208   N  MET H 201           
SHEET    6  HA 7 GLY H 226  HIS H 230 -1  O  PHE H 227   N  SER H 214           
SHEET    7  HA 7 MET H 180  ALA H 183 -1  O  PHE H 181   N  TYR H 228           
SHEET    1  HB 7 LYS H  81  SER H  83  0                                        
SHEET    2  HB 7 LEU H  64  ILE H  68 -1  O  VAL H  66   N  SER H  83           
SHEET    3  HB 7 GLN H  30  ARG H  35  0                                        
SHEET    4  HB 7 GLU H  39  LEU H  46  0                                        
SHEET    5  HB 7 TRP H  51  THR H  54 -1  O  LEU H  53   N  SER H  45           
SHEET    6  HB 7 ALA H 104  LEU H 108 -1  O  ALA H 104   N  THR H  54           
SHEET    7  HB 7 LEU H  85  ILE H  90 -1  N  GLU H  86   O  LYS H 107           
SHEET    1  HC 2 LEU H  60  TYR H  60A 0                                        
SHEET    2  HC 2 LYS H  60F ASN H  60G 0                                        
SSBOND   1 CYS H   42    CYS H   58                                             
SSBOND   2 CYS H  122    CYS L    1                                             
SSBOND   3 CYS H  168    CYS H  182                                             
SSBOND   4 CYS H  191    CYS H  220                                             
LINK         C   GLU I   8                 N   TYS I   9                        
LINK         C   TYS I   9                 N   LEU I  10                        
CISPEP   1 SER H   36A   PRO H   37          0        -0.79                     
SITE     1 AC1 23 HIS H  57  TRP H  60D TYR H  60A GLU H  97A                   
SITE     2 AC1 23 GLU H 146  ALA H 190  CYS H 191  GLU H 192                    
SITE     3 AC1 23 SER H 195  TRP H 215  GLY H 216  GLU H 217                    
SITE     4 AC1 23 GLY H 219  CYS H 220  HOH P   1  HOH P   2                    
SITE     5 AC1 23 HOH P   3  HOH P   4  HOH P   5  HOH P   6                    
SITE     6 AC1 23 HOH P   7  HOH S 517  HOH S 545                               
CRYST1   70.640   71.600   71.900  90.00 100.35  90.00 C 1 2 1       5          
ORIGX1      1.000000  0.000000  0.000000        0.00000                         
ORIGX2      0.000000  1.000000  0.000000        0.00000                         
ORIGX3      0.000000  0.000000  1.000000        0.00000                         
SCALE1      0.014156  0.000000  0.002585        0.00000                         
SCALE2      0.000000  0.013966  0.000000        0.00000                         
SCALE3      0.000000  0.000000  0.014138        0.00000